Synthetic biology for pharmaceutical drug discovery
JY Trosset, P Carbonell - Drug design, development and therapy, 2015 - Taylor & Francis
Synthetic biology (SB) is an emerging discipline, which is slowly reorienting the field of drug
discovery. For thousands of years, living organisms such as plants were the major source of …
discovery. For thousands of years, living organisms such as plants were the major source of …
A high-throughput mutational scan of an intrinsically disordered acidic transcriptional activation domain
Transcriptional activation domains are essential for gene regulation, but their intrinsic
disorder and low primary sequence conservation have made it difficult to identify the amino …
disorder and low primary sequence conservation have made it difficult to identify the amino …
A genome‐scale yeast library with inducible expression of individual genes
The ability to switch a gene from off to on and monitor dynamic changes provides a powerful
approach for probing gene function and elucidating causal regulatory relationships. Here …
approach for probing gene function and elucidating causal regulatory relationships. Here …
Synthetic biology tools for programming gene expression without nutritional perturbations in Saccharomyces cerevisiae
RS McIsaac, PA Gibney, SS Chandran… - Nucleic acids …, 2014 - academic.oup.com
A conditional gene expression system that is fast-acting, is tunable and achieves single-
gene specificity was recently developed for yeast. A gene placed directly downstream of a …
gene specificity was recently developed for yeast. A gene placed directly downstream of a …
Dual threshold optimization and network inference reveal convergent evidence from TF binding locations and TF perturbation responses
A high-confidence map of the direct, functional targets of each transcription factor (TF)
requires convergent evidence from independent sources. Two significant sources of …
requires convergent evidence from independent sources. Two significant sources of …
RNA polymerase errors cause splicing defects and can be regulated by differential expression of RNA polymerase subunits
LB Carey - Elife, 2015 - elifesciences.org
Errors during transcription may play an important role in determining cellular phenotypes:
the RNA polymerase error rate is> 4 orders of magnitude higher than that of DNA …
the RNA polymerase error rate is> 4 orders of magnitude higher than that of DNA …
A novel Candida glabrata doxycycline-inducible system for in vitro/in vivo use
S Schrevens, D Sanglard - FEMS Yeast Research, 2022 - academic.oup.com
Candida glabrata is an important pathogen causing superficial to invasive disease in
human. Conditional expression systems are helpful in addressing the function of genes and …
human. Conditional expression systems are helpful in addressing the function of genes and …
Map** transcription factor networks by comparing TF binding locations to TF perturbation responses
Background A transcription-factor (TF) network map indicates the direct, functional targets of
each TF--the genes it regulates by binding to their cis-regulatory DNA. Data on the genomic …
each TF--the genes it regulates by binding to their cis-regulatory DNA. Data on the genomic …
A deep mutational scan of an acidic activation domain
MV Staller, AS Holehouse, D Swain-Lenz, RK Das… - bioRxiv, 2017 - biorxiv.org
Transcriptional activation domains are intrinsically disordered peptides with little primary
sequence conservation. These properties have made it difficult to identify the sequence …
sequence conservation. These properties have made it difficult to identify the sequence …
[BOOK][B] Map** Transcription Factor Networks and Elucidating Their Biological Determinants
Y Kang - 2020 - search.proquest.com
A central goal in systems biology is to accurately map the transcription factor (TF) network of
a cell. Such a network map is a key component for many downstream applications, from …
a cell. Such a network map is a key component for many downstream applications, from …