Structural biology and molecular pharmacology of voltage-gated ion channels

J Huang, X Pan, N Yan - Nature Reviews Molecular Cell Biology, 2024 - nature.com
Voltage-gated ion channels (VGICs), including those for Na+, Ca2+ and K+, selectively
permeate ions across the cell membrane in response to changes in membrane potential …

[HTML][HTML] Single-particle cryo-EM at crystallographic resolution

Y Cheng - Cell, 2015 - cell.com
Until only a few years ago, single-particle electron cryo-microscopy (cryo-EM) was usually
not the first choice for many structural biologists due to its limited resolution in the range of …

A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis

J Zivanov, T Nakane, SHW Scheres - IUCrJ, 2019 - journals.iucr.org
A new method to estimate the trajectories of particle motion and the amount of cumulative
beam damage in electron cryo-microscopy (cryo-EM) single-particle analysis is presented …

[HTML][HTML] Cryo-electron microscopy for structural analysis of dynamic biological macromolecules

K Murata, M Wolf - Biochimica et Biophysica Acta (BBA)-General Subjects, 2018 - Elsevier
Background Since the introduction of what became today's standard for cryo-embedding of
biological macromolecules at native conditions more than 30 years ago, techniques and …

[HTML][HTML] An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology

RF Thompson, M Walker, CA Siebert, SP Muench… - Methods, 2016 - Elsevier
Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …

Quantification of gallium cryo-FIB milling damage in biological lamellae

BA Lucas, N Grigorieff - Proceedings of the National …, 2023 - National Acad Sciences
Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological
processes in their native, cellular environment at atomic resolution. However, few cells are …

Membranes under the magnetic lens: a dive into the diverse world of membrane protein structures using Cryo-EM

SJ Piper, RM Johnson, D Wootten, PM Sexton - Chemical reviews, 2022 - ACS Publications
Membrane proteins are highly diverse in both structure and function and can, therefore,
present different challenges for structure determination. They are biologically important for …

Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles

X Bai, IS Fernandez, G McMullan, SHW Scheres - elife, 2013 - elifesciences.org
Although electron cryo-microscopy (cryo-EM) single-particle analysis has become an
important tool for structural biology of large and flexible macro-molecular assemblies, the …

Structures of respiratory supercomplex I+ III2 reveal functional and conformational crosstalk

JA Letts, K Fiedorczuk, G Degliesposti, M Skehel… - Molecular cell, 2019 - cell.com
The mitochondrial electron transport chain complexes are organized into supercomplexes
(SCs) of defined stoichiometry, which have been proposed to regulate electron flux via …

Atomic model for the dimeric FO region of mitochondrial ATP synthase

H Guo, SA Bueler, JL Rubinstein - Science, 2017 - science.org
Mitochondrial adenosine triphosphate (ATP) synthase produces the majority of ATP in
eukaryotic cells, and its dimerization is necessary to create the inner membrane folds, or …