In the era of functional genomics, the need for tools to perform large-scale targeted and
random mutagenesis is increasing. A potential tool is Agrobacterium-mediated fungal …

Filamentous fungi are widely used in biotechnology as cell factories for the production of
chemicals, pharmaceuticals and enzymes. In order to improve their productivities, genetic …

Filamentous fungi have long been used for production of a range of valuable products; with
the advent of molecular biology, it became apparent that these fungi possess considerable …

The akuA gene encoding the Ku70 component of the nonhomologous end-joining
machinery was deleted in the opportunistic pathogen Aspergillus fumigatus. No obvious …

The study of gene function in filamentous fungi is a field of research that has made great
advances in very recent years. A number of transformation and gene manipulation strategies …

▪ Abstract Infections with mold pathogens have emerged as an increasing risk faced by
patients under sustained immunosuppression. Species of the Aspergillus family account for …

Commonly used protocols for the transformation of the dimorphic human fungal pathogen
Candida albicans rely on established methods for the yeast Saccharomyces cerevisiae. With …

In the koji molds Aspergillus sojae and Aspergillus oryzae, exogenous DNA is integrated in
the genome, in most cases irrespective of the sequence homology, suggesting that DNA …

The rapid accumulation of genomic sequences from a large number of eukaryotes, including
numerous filamentous fungi, has created a tremendous scientific potential, which can only …

Agrobacterium tumefaciens-mediated transformation has been successfully applied to the
wheat pathogen Mycosphaerella graminicola. Both protoplasts and intact cells have been …