Multi‐photon microscopy

J Sanderson - Current Protocols, 2023 - Wiley Online Library
In this series of papers on light microscopy imaging, we have covered the fundamentals of
microscopy, super‐resolution microscopy, and lightsheet microscopy. This last review …

Cell segmentation and tracking using CNN-based distance predictions and a graph-based matching strategy

T Scherr, K Löffler, M Böhland, R Mikut - PLoS One, 2020 - journals.plos.org
The accurate segmentation and tracking of cells in microscopy image sequences is an
important task in biomedical research, eg, for studying the development of tissues, organs or …

[HTML][HTML] u-track3D: Measuring, navigating, and validating dense particle trajectories in three dimensions

P Roudot, WR Legant, Q Zou, KM Dean, T Isogai… - Cell Reports …, 2023 - cell.com
We describe u-track3D, a software package that extends the versatile u-track framework
established in 2D to address the specific challenges of 3D particle tracking. First, we present …

A graph-based cell tracking algorithm with few manually tunable parameters and automated segmentation error correction

K Löffler, T Scherr, R Mikut - PloS one, 2021 - journals.plos.org
Automatic cell segmentation and tracking enables to gain quantitative insights into the
processes driving cell migration. To investigate new data with minimal manual effort, cell …

Neural circuit reorganisation after spinal cord injury in zebrafish

F El-Daher, CG Becker - Current opinion in genetics & development, 2020 - Elsevier
Spinal cord injuries disrupt signalling from the brain leading to loss of limb, locomotion,
sexual and bladder function, usually irreversible in humans. In zebrafish, recovery of …

Pax6 organizes the anterior eye segment by guiding two distinct neural crest waves

M Takamiya, J Stegmaier, AY Kobitski, B Schott… - PLoS …, 2020 - journals.plos.org
Cranial neural crest (NC) contributes to the develo** vertebrate eye. By multidimensional,
quantitative imaging, we traced the origin of the ocular NC cells to two distinct NC …

MorphoNet: an interactive online morphological browser to explore complex multi-scale data

B Leggio, J Laussu, A Carlier, C Godin… - Nature …, 2019 - nature.com
Powerful novel imaging and image-processing methods are revolutionizing many fields of
biology, at scales ranging from the molecule to the functional organ. To support this big-data …

Hemocytes in Drosophila melanogaster embryos move via heterogeneous anomalous diffusion

N Korabel, GD Clemente, D Han, F Feldman… - Communications …, 2022 - nature.com
Drosophila melanogaster hemocytes are highly motile cells that are crucial for successful
embryogenesis and have important roles in the organism's immunological response. Here …

Ventral striatal islands of Calleja neurons control grooming in mice

YF Zhang, L Vargas Cifuentes, KN Wright… - Nature …, 2021 - nature.com
The striatum comprises multiple subdivisions and neural circuits that differentially control
motor output. The islands of Calleja (IC) contain clusters of densely packed granule cells …

Ontology patterns for the representation of quality changes of cells in time

P Burek, N Scherf, H Herre - Journal of biomedical semantics, 2019 - Springer
Background Cell tracking experiments, based on time-lapse microscopy, have become an
important tool in biomedical research. The goal is the reconstruction of cell migration …