Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine
BACKGROUND Biomolecules can serve as natural labels for microscopy by measuring their
molecular vibration spectra in living cells and tissues. However, the transition from …
molecular vibration spectra in living cells and tissues. However, the transition from …
How it feels in a cell
Life emerges from thousands of biochemical processes occurring within a shared
intracellular environment. We have gained deep insights from in vitro reconstitution of …
intracellular environment. We have gained deep insights from in vitro reconstitution of …
Cholesteryl ester accumulation induced by PTEN loss and PI3K/AKT activation underlies human prostate cancer aggressiveness
Altered lipid metabolism is increasingly recognized as a signature of cancer cells. Enabled
by label-free Raman spectromicroscopy, we performed quantitative analysis of lipogenesis …
by label-free Raman spectromicroscopy, we performed quantitative analysis of lipogenesis …
Microsecond fingerprint stimulated Raman spectroscopic imaging by ultrafast tuning and spatial-spectral learning
Label-free vibrational imaging by stimulated Raman scattering (SRS) provides
unprecedented insight into real-time chemical distributions. Specifically, SRS in the …
unprecedented insight into real-time chemical distributions. Specifically, SRS in the …
Single cell optical imaging and spectroscopy
AS Stender, K Marchuk, C Liu, S Sander… - Chemical …, 2013 - ACS Publications
In his 1665 treatise, Micrographia, Robert Hooke described the many observations he had
made using a microscope, including compartment-like structures in cork samples that he …
made using a microscope, including compartment-like structures in cork samples that he …
Coherent Raman scattering microscopy in biology and medicine
Advancements in coherent Raman scattering (CRS) microscopy have enabled label-free
visualization and analysis of functional, endogenous biomolecules in living systems. When …
visualization and analysis of functional, endogenous biomolecules in living systems. When …
Quantitative vibrational imaging by hyperspectral stimulated Raman scattering microscopy and multivariate curve resolution analysis
Spectroscopic imaging has been an increasingly critical approach for unveiling specific
molecules in biological environments. Toward this goal, we demonstrate hyperspectral …
molecules in biological environments. Toward this goal, we demonstrate hyperspectral …
Fast vibrational imaging of single cells and tissues by stimulated Raman scattering microscopy
Conspectus Traditionally, molecules are analyzed in a test tube. Taking biochemistry as an
example, the majority of our knowledge about cellular content comes from analysis of fixed …
example, the majority of our knowledge about cellular content comes from analysis of fixed …
Quantum-enhanced continuous-wave stimulated Raman scattering spectroscopy
Stimulated Raman spectroscopy has become a powerful tool to study the spatiodynamics of
molecular bonds with high sensitivity, resolution, and speed. However, the sensitivity and …
molecular bonds with high sensitivity, resolution, and speed. However, the sensitivity and …
Open questions in lipid droplet biology
Y Ohsaki, M Suzuki, T Fujimoto - Chemistry & biology, 2014 - cell.com
Lipid droplets (LDs) have been the focus of intense research for the past decade because of
their active engagement in lipid metabolism and relationship with diseases. In contrast to …
their active engagement in lipid metabolism and relationship with diseases. In contrast to …