A fundamental prerequisite for the efficacy of cancer immunotherapy is the presence of
functional, antigen-specific T cells within the tumor. Neoantigen-directed therapy is a …

Sequencing the human genome empowers translational medicine, facilitating transcriptome‐
wide molecular diagnosis, pathway biology, and drug repositioning. Initially, microarrays are …

Early-life antibiotic exposure perturbs the intestinal microbiota and accelerates type 1
diabetes (T1D) development in the NOD mouse model. Here, we found that maternal cecal …

Nuclear depletion and cytoplasmic aggregation of the RNA-binding protein TDP-43 is the
hallmark of ALS, occurring in over 97% of cases. A key consequence of TDP-43 nuclear loss …

Most eukaryotic genes express alternative polyadenylation (APA) isoforms. A growing
number of RNA sequencing methods, especially those used for single-cell transcriptome …

Alternative splicing (AS) is a key post-transcriptional modification that helps in increasing
protein diversity. Almost 90% of the protein-coding genes in humans are known to undergo …

RNA-seq, including single cell RNA-seq (scRNA-seq), is plagued by insufficient sensitivity
and lack of precision. As a result, the full potential of (sc) RNA-seq is limited. Major factors in …

Pathological scarring resulting from traumas and wounds, such as hypertrophic scars and
keloids, pose significant aesthetic, functional and psychological challenges. This study …

The accuracy of methods for assembling transcripts from short-read RNA sequencing data is
limited by the lack of long-range information. Here we introduce Ladder-seq, an approach …