The Ecology of Acidobacteria: Moving beyond Genes and Genomes

AM Kielak, CC Barreto, GA Kowalchuk… - Frontiers in …, 2016 - frontiersin.org
The phylum Acidobacteria is one of the most widespread and abundant on the planet, yet
remarkably our knowledge of the role of these diverse organisms in the functioning of …

Metagenomics: application of genomics to uncultured microorganisms

J Handelsman - Microbiology and molecular biology reviews, 2004 - Am Soc Microbiol
Metagenomics (also referred to as environmental and community genomics) is the genomic
analysis of microorganisms by direct extraction and cloning of DNA from an assemblage of …

Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity

P Hugenholtz, BM Goebel, NR Pace - Journal of bacteriology, 1998 - Am Soc Microbiol
Our perspective on microbial diversity has improved enormously over the past few decades.
In large part this has been due to molecular phylogenetic studies that objectively relate …

The uncultured microbial majority

MS Rappé, SJ Giovannoni - Annual Reviews in Microbiology, 2003 - annualreviews.org
▪ Abstract Since the delineation of 12 bacterial phyla by comparative phylogenetic analyses
of 16S ribosomal RNA in 1987 knowledge of microbial diversity has expanded dramatically …

Identifying the dominant soil bacterial taxa in libraries of 16S rRNA and 16S rRNA genes

PH Janssen - Applied and environmental microbiology, 2006 - Am Soc Microbiol
In 1909, H. Joel Conn (25) expressed the hope that methods would soon be at hand by
which the significance of the different bacteria present in any soil could be determined …

Microbial diversity in soil: selection of microbial populations by plant and soil type and implications for disease suppressiveness

P Garbeva, JA Van Veen… - Annu. Rev. Phytopathol …, 2004 - annualreviews.org
▪ Abstract An increasing interest has emerged with respect to the importance of microbial
diversity in soil habitats. The extent of the diversity of microorganisms in soil is seen to be …

Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms

MR Rondon, PR August, AD Bettermann… - Applied and …, 2000 - Am Soc Microbiol
Recent progress in molecular microbial ecology has revealed that traditional culturing
methods fail to represent the scope of microbial diversity in nature, since only a small …

Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms

A Moter, UB Göbel - Journal of microbiological methods, 2000 - Elsevier
As a technique allowing simultaneous visualization, identification, enumeration and
localization of individual microbial cells, fluorescence in situ hybridization (FISH) is useful for …

Microbial population structures in soil particle size fractions of a long-term fertilizer field experiment

A Sessitsch, A Weilharter, MH Gerzabek… - Applied and …, 2001 - Am Soc Microbiol
Soil structure depends on the association between mineral soil particles (sand, silt, and clay)
and organic matter, in which aggregates of different size and stability are formed. Although …

Cultivating the uncultured

K Zengler, G Toledo, M Rappé… - Proceedings of the …, 2002 - National Acad Sciences
The recent application of molecular phylogeny to environmental samples has resulted in the
discovery of an abundance of unique and previously unrecognized microorganisms. The …