3D electron diffraction: the nanocrystallography revolution
Crystallography of nanocrystalline materials has witnessed a true revolution in the past 10
years, thanks to the introduction of protocols for 3D acquisition and analysis of electron …
years, thanks to the introduction of protocols for 3D acquisition and analysis of electron …
The cryo-EM method microcrystal electron diffraction (MicroED)
In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal
electron diffraction (MicroED). Since that time, data collection and analysis schemes have …
electron diffraction (MicroED). Since that time, data collection and analysis schemes have …
The CryoEM method MicroED as a powerful tool for small molecule structure determination
In the many scientific endeavors that are driven by organic chemistry, unambiguous
identification of small molecules is of paramount importance. Over the past 50 years, NMR …
identification of small molecules is of paramount importance. Over the past 50 years, NMR …
Structure of the toxic core of α-synuclein from invisible crystals
The protein α-synuclein is the main component of Lewy bodies, the neuron-associated
aggregates seen in Parkinson disease and other neurodegenerative pathologies. An 11 …
aggregates seen in Parkinson disease and other neurodegenerative pathologies. An 11 …
Protein data bank: a comprehensive review of 3D structure holdings and worldwide utilization by researchers, educators, and students
The Research Collaboratory for Structural Bioinformatics Protein Data Bank (RCSB PDB),
funded by the United States National Science Foundation, National Institutes of Health, and …
funded by the United States National Science Foundation, National Institutes of Health, and …
MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography
MyD88 and MAL are Toll-like receptor (TLR) adaptors that signal to induce pro-inflammatory
cytokine production. We previously observed that the TIR domain of MAL (MALTIR) forms …
cytokine production. We previously observed that the TIR domain of MAL (MALTIR) forms …
Analysis of global and site-specific radiation damage in cryo-EM
Micro-crystal electron diffraction (MicroED) combines the efficiency of electron scattering
with diffraction to allow structure determination from nano-sized crystalline samples in …
with diffraction to allow structure determination from nano-sized crystalline samples in …
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED
Traditionally, crystallographic analysis of macromolecules has depended on large, well-
ordered crystals, which often require significant effort to obtain. Even sizable crystals …
ordered crystals, which often require significant effort to obtain. Even sizable crystals …
Electron crystallography of ultrathin 3D protein crystals: atomic model with charges
K Yonekura, K Kato, M Ogasawara… - Proceedings of the …, 2015 - National Acad Sciences
Membrane proteins and macromolecular complexes often yield crystals too small or too thin
for even the modern synchrotron X-ray beam. Electron crystallography could provide a …
for even the modern synchrotron X-ray beam. Electron crystallography could provide a …
Serial protein crystallography in an electron microscope
Serial X-ray crystallography at free-electron lasers allows to solve biomolecular structures
from sub-micron-sized crystals. However, beam time at these facilities is scarce, and …
from sub-micron-sized crystals. However, beam time at these facilities is scarce, and …