For fluorogenic probes, Förster resonance energy transfer (FRET) is one of the most widely
used mechanisms to realize the detection of biochemical activities. Dark quenchers relax …

Sensing nucleic acids typically involves the recognition of a specific sequence and reporting
by, for example, a fluorogenic reaction yielding one activated dye molecule per detected …

Conformational dynamics of biomolecules are of fundamental importance for their function.
Single-molecule studies of Förster Resonance Energy Transfer (smFRET) between a …

DNA nanotechnology offers new biosensing approaches by templating different sensor and
transducer components. Here, we combine DNA origami nanoantennas with label-free …

Fluorogenic nucleic acid hybridization probes are widely used for detecting and quantifying
nucleic acids. The achieved sensitivity strongly depends on the contrast between a …

Single-molecule measurements of DNA hybridization kinetics are mostly performed on a
surface or inside a trap. Here we demonstrate a time-resolved, 3D single-molecule tracking …

Unsymmetrical dialkylamino‐substituted zinc azaphthalocyanine (AzaPc) exhibits unique
spectral and photophysical properties for dark quenchers of fluorescence in DNA …

Optical ruler methods employing multiple fluorescent labels offer great potential for
correlating distances among several sites, but are generally limited to interlabel distances …

Fluorescent dyes used for single-molecule spectroscopy can undergo millions of excitation-
emission cycles before photobleaching. Due to the upconcentration of light in a plasmonic …

A single chromophore can only emit a maximum of one single photon per excitation cycle.
This limitation results in a phenomenon commonly referred to as photon antibunching (pAB) …