A succession of technological advances over the past decade have enabled researchers to
chart maps of histone modifications and related chromatin structures with increasing …

Drastic epigenetic reprogramming takes place during preimplantation development, leading
to the conversion of terminally differentiated gametes to a totipotent embryo. Deficiencies in …

Chromatin profiling provides a versatile means to investigate functional genomic elements
and their regulation. However, current methods yield ensemble profiles that are insensitive …

RNA-guided genome editing with the CRISPR-Cas9 system has great potential for basic
and clinical research, but the determinants of targeting specificity and the extent of off-target …

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map
histone marks and transcription factor binding throughout the genome. Here we present …

Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to
mark the transcription start sites of active genes. Here, we show that H3K4me3 domains that …

Lifelong blood cell production is dependent on rare hematopoietic stem cells (HSCs) to
perpetually replenish mature cells via a series of lineage-restricted intermediates …

Combined chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) has
enabled genome-wide epigenetic profiling of numerous cell lines and tissue types. A major …

Differences in chromatin organization are key to the multiplicity of cell states that arise from a
single genetic background, yet the landscapes of in vivo tissues remain largely uncharted …

Imaging chromatin dynamics is crucial to understand genome organization and its role in
transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been …