New tools for recombinant protein production in Escherichia coli: A 5‐year update

GL Rosano, ES Morales, EA Ceccarelli - Protein science, 2019 - Wiley Online Library
The production of proteins in sufficient amounts is key for their study or use as biotherapeutic
agents. Escherichia coli is the host of choice for recombinant protein production given its fast …

Factors involved in heterologous expression of proteins in E. coli host

M Pouresmaeil, S Azizi-Dargahlou - Archives of Microbiology, 2023 - Springer
The production of recombinant proteins is one of the most significant achievements of
biotechnology in the last century. These proteins are produced in the eukaryotic or …

Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli

R Jiang, S Yuan, Y Zhou, Y Wei, F Li, M Wang… - Biotechnology …, 2024 - Elsevier
Protein expression is a critical process in diverse biological systems. For Escherichia coli, a
widely employed microbial host in industrial catalysis and healthcare, researchers often face …

Current trends in biopharmaceuticals production in Escherichia coli

L McElwain, K Phair, C Kealey, D Brady - Biotechnology letters, 2022 - Springer
Since the manufacture of the first biotech product for a fledgling biopharmaceutical industry
in 1982, Escherichia coli, has played an important role in the industrial production of …

Folding of heterologous proteins in bacterial cell factories: Cellular mechanisms and engineering strategies

Y Rong, SI Jensen, K Lindorff-Larsen… - Biotechnology Advances, 2023 - Elsevier
The expression of correctly folded and functional heterologous proteins is important in many
biotechnological production processes, whether it is enzymes, biopharmaceuticals or …

Evolution of insect innate immunity through domestication of bacterial toxins

KI Verster, G Cinege, Z Lipinszki… - Proceedings of the …, 2023 - National Acad Sciences
Toxin cargo genes are often horizontally transferred by phages between bacterial species
and are known to play an important role in the evolution of bacterial pathogenesis. Here, we …

Advances in Escherichia coli-Based Therapeutic Protein Expression: Mammalian Conversion, Continuous Manufacturing, and Cell-Free Production

SK Niazi, M Magoola - Biologics, 2023 - mdpi.com
Therapeutic proteins treat many acute and chronic diseases that were until recently
considered untreatable. However, their high development cost keeps them out of reach of …

[HTML][HTML] Experimental horizontal transfer of phage-derived genes to Drosophila confers innate immunity to parasitoids

RL Tarnopol, JA Tamsil, G Cinege, JH Ha, KI Verster… - Current Biology, 2025 - cell.com
Metazoan parasites have played a major role in sha** innate immunity in animals. Insect
hosts and parasitoid wasps are excellent models for illuminating how animal innate immune …

CRISPR-based construction of a BL21 (DE3)-derived variant strain library to rapidly improve recombinant protein production

ZJ Li, ZX Zhang, Y Xu, TQ Shi, C Ye, XM Sun… - ACS Synthetic …, 2021 - ACS Publications
Escherichia coli BL21 (DE3) is the most widely used host for recombinant protein
expression. However, not every protein can be highly expressed in BL21 (DE3), so …

Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm

B Laurinyecz, V Vedelek, AL Kovács, K Szilasi… - PLoS …, 2019 - journals.plos.org
Drosophila melanogaster sperm reach an extraordinary long size, 1.8 mm, by the end of
spermatogenesis. The mitochondrial derivatives run along the entire flagellum and provide …