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Rationalizing membrane protein overexpression
Functional and structural studies of membrane proteins usually require overexpression of
the proteins in question. Often, however, the 'trial and error'approaches that are mainly used …
the proteins in question. Often, however, the 'trial and error'approaches that are mainly used …
[HTML][HTML] Microbial expression systems for membrane proteins
Despite many high-profile successes, recombinant membrane protein production remains a
technical challenge; it is still the case that many fewer membrane protein structures have …
technical challenge; it is still the case that many fewer membrane protein structures have …
Rice NON-YELLOW COLORING1 is involved in light-harvesting complex II and grana degradation during leaf senescence
Chlorophyll degradation is an aspect of leaf senescence, which is an active process to
salvage nutrients from old tissues. non-yellow coloring1 (nyc1) is a rice (Oryza sativa) stay …
salvage nutrients from old tissues. non-yellow coloring1 (nyc1) is a rice (Oryza sativa) stay …
Optimization of membrane protein overexpression and purification using GFP fusions
Optimizing conditions for the overexpression and purification of membrane proteins for
functional and structural studies is usually a laborious and time-consuming process. This …
functional and structural studies is usually a laborious and time-consuming process. This …
LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake
Tricyclic antidepressants exert their pharmacological effect—inhibiting the reuptake of
serotonin, norepinephrine, and dopamine—by directly blocking neurotransmitter …
serotonin, norepinephrine, and dopamine—by directly blocking neurotransmitter …
Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21 (DE3)
Escherichia coli BL21 (DE3) is widely used to overexpress proteins. In this overexpression
host, the gene encoding the target protein is located on a plasmid and is under control of the …
host, the gene encoding the target protein is located on a plasmid and is under control of the …
High level cell‐free expression and specific labeling of integral membrane proteins
C Klammt, F Löhr, B Schäfer, W Haase… - European Journal of …, 2004 - Wiley Online Library
We demonstrate the high level expression of integral membrane proteins (IMPs) in a cell‐
free coupled transcription/translation system using a modified Escherichia coli S30 extract …
free coupled transcription/translation system using a modified Escherichia coli S30 extract …
Structure and mechanism of a bacterial sodium-dependent dicarboxylate transporter
In human cells, cytosolic citrate is a chief precursor for the synthesis of fatty acids,
triacylglycerols, cholesterol and low-density lipoprotein. Cytosolic citrate further regulates …
triacylglycerols, cholesterol and low-density lipoprotein. Cytosolic citrate further regulates …
Identification and characterization of a bacterial hydrosulphide ion channel
The hydrosulphide ion (HS−) and its undissociated form, hydrogen sulphide (H2S), which
are believed to have been critical to the origin of life on Earth, remain important in …
are believed to have been critical to the origin of life on Earth, remain important in …
Quality control of overexpressed membrane proteins
Overexpression of membrane proteins in Escherichia coli frequently leads to the formation of
aggregates or inclusion bodies, which is undesirable for most studies. Ideally, one would …
aggregates or inclusion bodies, which is undesirable for most studies. Ideally, one would …