CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering

DJ Dickinson, B Goldstein - Genetics, 2016 - academic.oup.com
The advent of genome editing techniques based on the clustered regularly interspersed
short palindromic repeats (CRISPR)–Cas9 system has revolutionized research in the …

The Caenorhabditis elegans Transgenic Toolbox

J Nance, C Frøkjær-Jensen - Genetics, 2019 - academic.oup.com
The power of any genetic model organism is derived, in part, from the ease with which gene
expression can be manipulated. The short generation time and invariant developmental …

Streamlined genome engineering with a self-excising drug selection cassette

DJ Dickinson, AM Pani, JK Heppert, CD Higgins… - Genetics, 2015 - academic.oup.com
A central goal in the development of genome engineering technology is to reduce the time
and labor required to produce custom genome modifications. Here we describe a new …

Regulation of lifespan by neural excitation and REST

JM Zullo, D Drake, L Aron, P O'Hern, SC Dhamne… - Nature, 2019 - nature.com
The mechanisms that extend lifespan in humans are poorly understood. Here we show that
extended longevity in humans is associated with a distinct transcriptome signature in the …

Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination

DJ Dickinson, JD Ward, DJ Reiner, B Goldstein - Nature methods, 2013 - nature.com
Study of the nematode Caenorhabditis elegans has provided important insights in a wide
range of fields in biology. The ability to precisely modify genomes is critical to fully realize …

Heritable genome editing in C. elegans via a CRISPR-Cas9 system

AE Friedland, YB Tzur, KM Esvelt, MP Colaiácovo… - Nature …, 2013 - nature.com
We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-
associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans …

An expanded auxin-inducible degron toolkit for Caenorhabditis elegans

GE Ashley, T Duong, MT Levenson, MAQ Martinez… - Genetics, 2021 - academic.oup.com
The auxin-inducible degron (AID) system has emerged as a powerful tool to conditionally
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to …

[HTML][HTML] piRNAs can trigger a multigenerational epigenetic memory in the germline of C. elegans

A Ashe, A Sapetschnig, EM Weick, J Mitchell… - Cell, 2012 - cell.com
Transgenerational effects have wide-ranging implications for human health, biological
adaptation, and evolution; however, their mechanisms and biology remain poorly …

Mammalian TRP ion channels are insensitive to membrane stretch

Y Nikolaev, CD Cox, P Ridone, PR Rohde… - Biophysical …, 2020 - cell.com
TRP channels of the transient receptor potential ion channel superfamily play an important
role in a wide variety of mechanosensory processes, including touch sensation, pain, blood …

The piRNA targeting rules and the resistance to piRNA silencing in endogenous genes

D Zhang, S Tu, M Stubna, WS Wu, WC Huang, Z Weng… - Science, 2018 - science.org
Piwi-interacting RNAs (piRNAs) silence transposons to safeguard genome integrity in
animals. However, the functions of the many piRNAs that do not map to transposons remain …