CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering
The advent of genome editing techniques based on the clustered regularly interspersed
short palindromic repeats (CRISPR)–Cas9 system has revolutionized research in the …
short palindromic repeats (CRISPR)–Cas9 system has revolutionized research in the …
The Caenorhabditis elegans Transgenic Toolbox
J Nance, C Frøkjær-Jensen - Genetics, 2019 - academic.oup.com
The power of any genetic model organism is derived, in part, from the ease with which gene
expression can be manipulated. The short generation time and invariant developmental …
expression can be manipulated. The short generation time and invariant developmental …
Streamlined genome engineering with a self-excising drug selection cassette
A central goal in the development of genome engineering technology is to reduce the time
and labor required to produce custom genome modifications. Here we describe a new …
and labor required to produce custom genome modifications. Here we describe a new …
Regulation of lifespan by neural excitation and REST
The mechanisms that extend lifespan in humans are poorly understood. Here we show that
extended longevity in humans is associated with a distinct transcriptome signature in the …
extended longevity in humans is associated with a distinct transcriptome signature in the …
Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination
Study of the nematode Caenorhabditis elegans has provided important insights in a wide
range of fields in biology. The ability to precisely modify genomes is critical to fully realize …
range of fields in biology. The ability to precisely modify genomes is critical to fully realize …
Heritable genome editing in C. elegans via a CRISPR-Cas9 system
We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-
associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans …
associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans …
An expanded auxin-inducible degron toolkit for Caenorhabditis elegans
The auxin-inducible degron (AID) system has emerged as a powerful tool to conditionally
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to …
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to …
[HTML][HTML] piRNAs can trigger a multigenerational epigenetic memory in the germline of C. elegans
A Ashe, A Sapetschnig, EM Weick, J Mitchell… - Cell, 2012 - cell.com
Transgenerational effects have wide-ranging implications for human health, biological
adaptation, and evolution; however, their mechanisms and biology remain poorly …
adaptation, and evolution; however, their mechanisms and biology remain poorly …
Mammalian TRP ion channels are insensitive to membrane stretch
TRP channels of the transient receptor potential ion channel superfamily play an important
role in a wide variety of mechanosensory processes, including touch sensation, pain, blood …
role in a wide variety of mechanosensory processes, including touch sensation, pain, blood …
The piRNA targeting rules and the resistance to piRNA silencing in endogenous genes
Piwi-interacting RNAs (piRNAs) silence transposons to safeguard genome integrity in
animals. However, the functions of the many piRNAs that do not map to transposons remain …
animals. However, the functions of the many piRNAs that do not map to transposons remain …