Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …

Cas9 and Cas12a are multidomain CRISPR‐associated nucleases that can be programmed
with a guide RNA to bind and cleave complementary DNA targets. The guide RNA …

Summary CRISPR-Cas12a (Cpf1) is an RNA-guided DNA-cutting nuclease that has been
repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target …

Food analysis to ensure food safety and quality are relevant to all countries. This study
aimed to develop a detection technique by combining recombinase polymerase …

Due to their biotechnological relevance as well as their importance as disease agents,
filamentous fungi and oomycetes have been prime candidates for genetic selection and in …

The Sc2. 0 project is building a eukaryotic synthetic genome from scratch. A major milestone
has been achieved with all individual Sc2. 0 chromosomes assembled. Here, we describe …

CRISPR and CRISPR-associated (Cas) protein, as components of microbial adaptive
immune system, allows biologists to edit genomic DNA in a precise and specific way …

The non-conventional oleaginous yeast Yarrowia lipolytica shows great industrial promise. It
naturally produces certain compounds of interest but can also artificially generate non-native …

Streptomyces has a strong capability for producing a large number of bioactive natural
products and remains invaluable as a source for the discovery of novel drug leads. Although …

Background Agri-food safety has been considered as one of the most important public
concerns worldwide. From farm to table, food crops and foods are extremely vulnerable to …