Negamycin is a natural product with broad-spectrum antibacterial activity and efficacy in
animal models of infection. Although its precise mechanism of action has yet to be …

Nonsense suppressors (NonSups) induce “readthrough”, ie, the selection of near cognate
tRNAs at premature termination codons and insertion of the corresponding amino acid into …

RNA-protein complexes (RNPs) are essential components in a variety of cellular processes,
and oftentimes exhibit complex structures and show mechanisms that are highly dynamic in …

N6-methyladenosine (m6A) is the most abundant and well understood modification in
eukaryotic mRNA and was first identified in polyadenylated parts of the mRNA. The distinct …

Inhibition of protein synthesis is one of the most common modes of action for medically
useful antibiotics. This thesis presents the mechanistic studies of two chemically distinct …

The post-transcriptional modification of the canonical nucleoside uridine into its rotational
isomer pseudouridine occurs in non-coding as well as coding RNA and is the most …

Protein synthesis occurs on the ribosome, a sophisticated cellular machine that decodes the
genetic message stored in mRNA. Elongation factor thermo unstable (EF-Tu), a three …

The result of nonsense mutation is premature stop-codon (PTC) in an open reading frame of
a gene, resulting in truncated, inactive protein product. This leads to many diseases like …

Single-molecule techniques have been used to uncover the biophysical mechanisms of
antimicrobial targets and their mechanisms of inhibition, and could make important …

The aim of this project was, primarily, to clone, express and investigate the function of a cfr-
like gene from Clostridium sporogenes (clcs) in E. coli and, subsequently, to investigate the …