VIGS vectors for gene silencing: many targets, many tools

D Robertson - Annu. Rev. Plant Biol., 2004 - annualreviews.org
▪ Abstract The discovery that plants recognize and degrade invading viral RNA caused a
paradigm shift in our understanding of viral/host interactions. Combined with the discovery …

Microparticle bombardment as a tool in plant science and agricultural biotechnology

NJ Taylor, CM Fauquet - DNA and cell biology, 2002 - liebertpub.com
Microparticle bombardment technology has evolved as a method for delivering exogenous
nucleic acids into plant cells and is a commonly employed technique in plant science …

Simple, but not branched, plasmodesmata allow the nonspecific trafficking of proteins in develo** tobacco leaves

KJ Oparka, AG Roberts, P Boevink, S Santa Cruz… - Cell, 1999 - cell.com
Leaves undergo a sink–source transition during which a physiological change occurs from
carbon import to export. In sink leaves, biolistic bombardment of plasmids encoding GFP …

Multiple C2 domains and transmembrane region proteins (MCTP s) tether membranes at plasmodesmata

ML Brault, JD Petit, F Immel, WJ Nicolas, M Glavier… - EMBO …, 2019 - embopress.org
In eukaryotes, membrane contact sites (MCS) allow direct communication between
organelles. Plants have evolved a unique type of MCS, inside intercellular pores, the …

Chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated

S Harpaz-Saad, T Azoulay, T Arazi… - The Plant …, 2007 - academic.oup.com
Chlorophyll is a central player in harvesting light energy for photosynthesis, yet the rate-
limiting steps of chlorophyll catabolism and the regulation of the catabolic enzymes remain …

The potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eIF4E in cell‐to‐cell trafficking

Z Gao, E Johansen, S Eyers, CL Thomas… - The Plant …, 2004 - Wiley Online Library
From the characterization of the recessive resistance gene, sbm1, in pea we have identified
the eukaryotic translation initiation factor, eIF4E, as a susceptibility factor required for …

The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development

YM Shiboleth, E Haronsky, D Leibman, T Arazi… - Journal of …, 2007 - Am Soc Microbiol
The helper component-proteinase (HC-Pro) protein of potyviruses is a suppressor of gene
silencing and has been shown to elicit plant developmental-defect-like symptoms. In …

Mutations in Potato virus Y Genome-Linked Protein Determine Virulence Toward Recessive Resistances in Capsicum annuum and Lycopersicon hirsutum

B Moury, C Morel, E Johansen… - Molecular Plant …, 2004 - Am Phytopath Society
The recessive resistance genes pot-1 and pvr2 in Lycopersicon hirsutum and Capsicum
annuum, respectively, control Potato virus Y (PVY) accumulation in the inoculated leaves …

Mutations in the HC-Pro gene of zucchini yellow mosaic potyvirus: effects on aphid transmission and binding to purified virions.

Y Peng, D Kadoury, A Gal-On… - Journal of General …, 1998 - microbiologyresearch.org
Transmission of zucchini yellow mosaic virus (ZYMV) by aphids was examined by
introducing mutations within the highly conserved proline-threonine-lysine (PTK) motif of the …

Characterization of Cucumber mosaic virus

T Canto, DAM Prior, KH Hellwald, KJ Oparka… - Virology, 1997 - Elsevier
cDNA clones of cucumber mosaic virus (CMV) RNA 3 were modified to express the jellyfish
green fluorescent protein (GFP) in place of the 3a movement protein (MP) or coat protein …