Single-cell proteomics enabled by next-generation sequencing or mass spectrometry

HM Bennett, W Stephenson, CM Rose, S Darmanis - Nature Methods, 2023‏ - nature.com
In the last decade, single-cell RNA sequencing routinely performed on large numbers of
single cells has greatly advanced our understanding of the underlying heterogeneity of …

Molecular mechanisms of environmental exposures and human disease

H Wu, CM Eckhardt, AA Baccarelli - Nature reviews genetics, 2023‏ - nature.com
A substantial proportion of disease risk for common complex disorders is attributable to
environmental exposures and pollutants. An appreciation of how environmental pollutants …

Molecular responses to acute exercise and their relevance for adaptations in skeletal muscle to exercise training

B Egan, AP Sharples - Physiological Reviews, 2023‏ - journals.physiology.org
Repeated, episodic bouts of skeletal muscle contraction undertaken frequently as structured
exercise training are a potent stimulus for physiological adaptation in many organs …

The diversification of methods for studying cell–cell interactions and communication

E Armingol, HM Baghdassarian, NE Lewis - Nature Reviews Genetics, 2024‏ - nature.com
No cell lives in a vacuum, and the molecular interactions between cells define most
phenotypes. Transcriptomics provides rich information to infer cell–cell interactions and …

Map** the proteo-genomic convergence of human diseases

M Pietzner, E Wheeler, J Carrasco-Zanini, A Cortes… - Science, 2021‏ - science.org
INTRODUCTION Proteins are essential functional units of the human body and represent
the largest class of drug targets. RATIONALE Broad-capture proteomics has the potential to …

Deciphering cell–cell interactions and communication from gene expression

E Armingol, A Officer, O Harismendy… - Nature Reviews …, 2021‏ - nature.com
Cell–cell interactions orchestrate organismal development, homeostasis and single-cell
functions. When cells do not properly interact or improperly decode molecular messages …

Unbiased spatial proteomics with single-cell resolution in tissues

A Mund, AD Brunner, M Mann - Molecular cell, 2022‏ - cell.com
Mass spectrometry (MS)-based proteomics has become a powerful technology to quantify
the entire complement of proteins in cells or tissues. Here, we review challenges and recent …

Cancer proteogenomics: current impact and future prospects

DR Mani, K Krug, B Zhang, S Satpathy… - Nature Reviews …, 2022‏ - nature.com
Genomic analyses in cancer have been enormously impactful, leading to the identification of
driver mutations and development of targeted therapies. But the functions of the vast majority …

Single-cell proteomic and transcriptomic analysis of macrophage heterogeneity using SCoPE2

H Specht, E Emmott, AA Petelski, RG Huffman… - Genome biology, 2021‏ - Springer
Background Macrophages are innate immune cells with diverse functional and molecular
phenotypes. This diversity is largely unexplored at the level of single-cell proteomes …

Ultra‐high sensitivity mass spectrometry quantifies single‐cell proteome changes upon perturbation

AD Brunner, M Thielert, C Vasilopoulou… - Molecular systems …, 2022‏ - embopress.org
Single‐cell technologies are revolutionizing biology but are today mainly limited to imaging
and deep sequencing. However, proteins are the main drivers of cellular function and in …