Noninvasive morpho-molecular imaging reveals early therapy-induced senescence in human cancer cells

A Bresci, JH Kim, S Ghislanzoni, F Manetti, L Wu… - Science …, 2023‏ - science.org
Anticancer therapy screening in vitro identifies additional treatments and improves clinical
outcomes. Systematically, although most tested cells respond to cues with apoptosis, an …

Raman spectroscopy and AI applications in cancer grading. An overview

PM Conforti, G Lazzini, P Russo, M D'Acunto - IEEE Access, 2024‏ - ieeexplore.ieee.org
Raman spectroscopy (RS) is a label-free molecular vibrational spectroscopy technique that
is able to identify the molecular fingerprint of various samples making use of the inelastic …

Full-spectrum CARS microscopy of cells and tissues with ultrashort white-light continuum pulses

F Vernuccio, R Vanna, C Ceconello… - The Journal of …, 2023‏ - ACS Publications
Coherent anti-Stokes Raman scattering (CARS) microscopy is an emerging nonlinear
vibrational imaging technique that delivers label-free chemical maps of cells and tissues. In …

High-throughput multimodal wide-field Fourier-transform Raman microscope

B Ardini, A Bassi, A Candeo, A Genco, C Trovatello… - Optica, 2023‏ - opg.optica.org
Raman microscopy is a powerful analytical technique for materials and life sciences that
enables map** the spatial distribution of the chemical composition of a sample. State-of …

Fingerprint multiplex CARS at high speed based on supercontinuum generation in bulk media and deep learning spectral denoising

F Vernuccio, A Bresci, B Talone, A de la Cadena… - Optics …, 2022‏ - opg.optica.org
We introduce a broadband coherent anti-Stokes Raman scattering (CARS) microscope
based on a 2-MHz repetition rate ytterbium laser generating 1035-nm high-energy (≈ µJ …

[HTML][HTML] Broadband stimulated Raman imaging based on multi-channel lock-in detection for spectral histopathology

A De la Cadena, F Vernuccio, A Ragni, G Sciortino… - Apl Photonics, 2022‏ - pubs.aip.org
Spontaneous Raman microscopy reveals the chemical composition of a sample in a label-
free and non-invasive fashion by directly measuring the vibrational spectra of molecules …

Raman micro-spectroscopy reveals the spatial distribution of fumarate in cells and tissues

M Kamp, J Surmacki, M Segarra Mondejar… - Nature …, 2024‏ - nature.com
Aberrantly accumulated metabolites elicit intra-and inter-cellular pro-oncogenic cascades,
yet current measurement methods require sample perturbation/disruption and lack spatio …

Self-referencing for quasi shot-noise-limited widefield transient microscopy

M Hörmann, F Visentin, SK Chakraborty, B Nayak… - Optics …, 2024‏ - opg.optica.org
Many applications of ultrafast and nonlinear optical microscopy require the measurement of
small differential signals over large fields-of-view. Widefield configurations drastically reduce …

A dual-modal, label-free Raman imaging method for rapid virtual staining of large-area breast cancer tissue sections

W Mo, Q Ke, Q Yang, M Zhou, G **e, D Qi… - Analytical …, 2024‏ - ACS Publications
As one of the most common cancers, accurate, rapid, and simple histopathological
diagnosis is very important for breast cancer. Raman imaging is a powerful technique for …

Simultaneous label-free autofluorescence multi-harmonic microscopy driven by the supercontinuum generated from a bulk nonlinear crystal

A De la Cadena, J Park, KF Tehrani… - Biomedical Optics …, 2024‏ - opg.optica.org
Nonlinear microscopy encompasses several imaging techniques that leverage laser
technology to probe intrinsic molecules of biological specimens. These native molecules …