Recombinant protein scaffolds for tissue engineering

JA Werkmeister, JAM Ramshaw - Biomedical materials, 2012 - iopscience.iop.org
New biological materials for tissue engineering are now being developed using common
genetic engineering capabilities to clone and express a variety of genetic elements that …

Recombinant technology in the development of materials and systems for soft‐tissue repair

A Girotti, D Orbanic, A Ibáñez‐Fonseca… - Advanced …, 2015 - Wiley Online Library
The field of biomedicine is constantly investing significant research efforts in order to gain a
more in‐depth understanding of the mechanisms that govern the function of body …

Alginate/human elastin-like polypeptide composite films with antioxidant properties for potential wound healing application

C Bergonzi, GG d'Ayala, L Elviri, P Laurienzo… - International journal of …, 2020 - Elsevier
In this contribution we describe the preparation and characterization of a series of cross-
linked films based on the combination of an elastin-derived biomimetic polypeptide (Human …

[HTML][HTML] Nanoscale bilirubin analysis in translational research and precision medicine by the recombinant protein HUG

P Sist, F Tramer, A Bandiera, R Urbani… - International Journal of …, 2023 - mdpi.com
Bilirubin is a toxicological biomarker for hemolysis and liver diseases. The current
automated diazo method used in clinical chemistry has limited applicability in rodent models …

Physicochemical Characterization of a Biomimetic, Elastin-Inspired Polypeptide with Enhanced Thermoresponsive Properties and Improved Cell Adhesion

A Bandiera, L Colomina-Alfaro, P Sist… - …, 2023 - ACS Publications
Genetic engineering allows fine-tuning and controlling protein properties, thus exploiting the
new derivatives to obtain novel materials and systems with improved capacity to actively …

Macromolecular and solution properties of the recombinant fusion protein HUG

P Sist, A Bandiera, R Urbani, S Passamonti - Biomacromolecules, 2022 - ACS Publications
The recombinant fusion protein HELP-UnaG (HUG) is a bifunctional product that exhibits
human elastin-like polypeptide (HELP)-specific thermal behavior, defined as a reverse …

A Versatile Elastin‐Like Carrier for Bioactive Antimicrobial Peptide Production and Delivery

L Colomina–Alfaro, P Sist, S Marchesan… - Macromolecular …, 2024 - Wiley Online Library
Elastin‐like polypeptides are biotechnological protein and peptide carriers that offer a vast
scope of applicability. This work aims to build a model for the expression of antimicrobial …

An optimized table-top small-angle X-ray scattering set-up for the nanoscale structural analysis of soft matter

T Sibillano, L De Caro, D Altamura, D Siliqi… - Scientific reports, 2014 - nature.com
The paper shows how a table top superbright microfocus laboratory X-ray source and an
innovative restoring-data algorithm, used in combination, allow to analyze the super …

[HTML][HTML] Standardized lab-scale production of the recombinant fusion protein HUG for the nanoscale analysis of bilirubin

P Sist, S Saeed, F Tramer, A Bandiera, S Passamonti - MethodsX, 2024 - Elsevier
The recombinant bifunctional protein HELP-UnaG (HUG) is a fusion product of the Human
Elastin-like Polypeptide (HELP) with the bilirubin-binding fluorescent protein UnaG. HUG is …

Structural and hydrodynamic analysis of a novel drug delivery vector: ELP [V5G3A2-150]

DF Lyons, V Le, GL Bidwell, WH Kramer, EA Lewis… - Biophysical …, 2013 - cell.com
The therapeutic potential of elastin-like polypeptide (ELP) conjugated to therapeutic
compounds is currently being investigated as an approach to target drugs to solid tumors …