Emerging diversity in lipid–protein interactions

V Corradi, BI Sejdiu, H Mesa-Galloso… - Chemical …, 2019 - ACS Publications
Membrane lipids interact with proteins in a variety of ways, ranging from providing a stable
membrane environment for proteins to being embedded in to detailed roles in complicated …

Folding and misfolding of human membrane proteins in health and disease: from single molecules to cellular proteostasis

JT Marinko, H Huang, WD Penn, JA Capra… - Chemical …, 2019 - ACS Publications
Advances over the past 25 years have revealed much about how the structural properties of
membranes and associated proteins are linked to the thermodynamics and kinetics of …

Mass spectrometry methods for measuring protein stability

DD Vallejo, C Rojas Ramírez, KF Parson, Y Han… - Chemical …, 2022 - ACS Publications
Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …

Combining native and 'omics' mass spectrometry to identify endogenous ligands bound to membrane proteins

J Gault, I Liko, M Landreh, D Shutin, JR Bolla… - Nature …, 2020 - nature.com
Ligands bound to protein assemblies provide critical information for function, yet are often
difficult to capture and define. Here we develop a top-down method,'nativeomics', unifying …

Membrane protein–lipid interactions probed using mass spectrometry

JR Bolla, MT Agasid, S Mehmood… - Annual Review of …, 2019 - annualreviews.org
Membrane proteins that exist in lipid bilayers are not isolated molecular entities. The lipid
molecules that surround them play crucial roles in maintaining their full structural and …

The effects of sodium ions on ligand binding and conformational states of G protein-coupled receptors—insights from mass spectrometry

MT Agasid, L Sørensen, LH Urner, J Yan… - Journal of the …, 2021 - ACS Publications
The use of mass spectrometry to investigate proteins is now well established and provides
invaluable information for both soluble and membrane protein assemblies. Maintaining …

Surface-induced dissociation of noncovalent protein complexes in an extended mass range orbitrap mass spectrometer

ZL VanAernum, JD Gilbert, ME Belov… - Analytical …, 2019 - ACS Publications
Native mass spectrometry continues to develop as a significant complement to traditional
structural biology techniques. Within native mass spectrometry (MS), surface-induced …

Surface-induced dissociation: an effective method for characterization of protein quaternary structure

AQ Stiving, ZL VanAernum, F Busch… - Analytical …, 2018 - ACS Publications
METHODS Herein we describe alternative dissociation methods commonly employed within
mass spectrometry with an emphasis on the outcomes of probing native protein complexes …

Targeting bacterial phospholipids and their synthesis pathways for antibiotic discovery

M Song, S Chen, W Lin, K Zhu - Progress in Lipid Research, 2024 - Elsevier
Bacterial infections in humans and animals caused by multidrug-resistant (MDR) pathogens
pose a serious threat to public health. New antibacterial targets are extremely urgent to …

Infrared Photoactivation Enables Improved Native Top-Down Mass Spectrometry of Transmembrane Proteins

BR Juliano, JW Keating, BT Ruotolo - Analytical chemistry, 2023 - ACS Publications
Membrane proteins are often challenging targets for native top-down mass spectrometry
experimentation. The requisite use of membrane mimetics to solubilize such proteins …