Mechanism-based tuning of a LOV domain photoreceptor

BD Zoltowski, B Vaccaro, BR Crane - Nature chemical biology, 2009 - nature.com
Phototropin-like LOV domains form a cysteinyl-flavin adduct in response to blue light but
show considerable variation in output signal and the lifetime of the photo-adduct signaling …

Engineering of LOV-domains for their use as protein tags

SG Kaya, A Hovan, MW Fraaije - Archives of Biochemistry and Biophysics, 2024 - Elsevier
Abstract Light-Oxygen-Voltage (LOV) domains are the protein-based light switches used in
nature to trigger and regulate various processes. They allow light signals to be converted …

Factors that control the chemistry of the LOV domain photocycle

JP Zayner, TR Sosnick - PloS one, 2014 - journals.plos.org
Algae, plants, bacteria and fungi contain Light-Oxygen-Voltage (LOV) domains that function
as blue light sensors to control cellular responses to light. All LOV domains contain a bound …

Primary reactions of the LOV2 domain of phototropin studied with ultrafast mid-infrared spectroscopy and quantum chemistry

MTA Alexandre, T Domratcheva, C Bonetti… - Biophysical journal, 2009 - cell.com
Phototropins, major blue-light receptors in plants, are sensitive to blue light through a pair of
flavin mononucleotide (FMN)-binding light oxygen and voltage (LOV) domains, LOV1 and …

Unfolding of the C-terminal Jα helix in the LOV2 photoreceptor domain observed by time-resolved vibrational spectroscopy

PE Konold, T Mathes, J Weiβenborn… - The Journal of …, 2016 - ACS Publications
Light-triggered reactions of biological photoreceptors have gained immense attention for
their role as molecular switches in their native organisms and for optogenetic application …

Femtosecond-to-nanosecond dynamics of flavin mononucleotide monitored by stimulated Raman spectroscopy and simulations

PC Andrikopoulos, Y Liu, A Picchiotti… - Physical Chemistry …, 2020 - pubs.rsc.org
Flavin mononucleotide (FMN) belongs to the large family of flavins, ubiquitous yellow-
coloured biological chromophores that contain an isoalloxazine ring system. As a cofactor in …

[HTML][HTML] Conformational heterogeneity and propagation of structural changes in the LOV2/Jα domain from Avena sativa phototropin 1 as recorded by temperature …

MTA Alexandre, R van Grondelle, KJ Hellingwerf… - Biophysical journal, 2009 - cell.com
Phototropins control phototropism, chloroplast movement, stomatal opening, and leaf
expansion in plants. Phototropin 1 (phot1) is composed of a kinase domain linked to two …

Modulating LOV domain photodynamics with a residue alteration outside the chromophore binding site

SH Song, PL Freddolino, AI Nash, EC Carroll… - Biochemistry, 2011 - ACS Publications
Phototropins, a class of light-activated protein kinases, are essential for several blue light
responses in plants and algae, including phototropism. These proteins contain two internal …

Influence of the LOV domain on low-lying excited states of flavin: a combined quantum-mechanics/molecular-mechanics investigation

S Salzmann, MR Silva-Junior, W Thiel… - The Journal of …, 2009 - ACS Publications
The ground and low-lying excited states of flavin mononucleotide (FMN) in the light, oxygen,
and voltage sensitive (LOV) domain of the blue-light photosensor YtvA of Bacillus subtilis …

Photoreaction of plant and DASH cryptochromes probed by infrared spectroscopy: the neutral radical state of flavoproteins

D Immeln, R Pokorny, E Herman, J Moldt… - The Journal of …, 2010 - ACS Publications
Flavoprotein radicals are important intermediates in many biochemical processes. In the
blue light sensor plant cryptochrome, the radical state acts as a signaling state. An isolation …