It has long been axiomatic that a protein's structure determines its function. Intrinsically
disordered proteins (IDPs) and disordered protein regions (IDRs) defy this structure …

Flow cytometry (FCM) is a sophisticated technique that works on the principle of light
scattering and fluorescence emission by the specific fluorescent probe-labeled cells as they …

Aberrantly processed or mutant proteins misfold and assemble into a variety of soluble
oligomers and insoluble aggregates, a process that is associated with an increasing number …

One of the grand challenges of biophysical chemistry is to understand the principles that
govern protein misfolding and aggregation, which is a highly complex process that is …

Aberrant phase separation of globular proteins is associated with many diseases. Here, we
use a model protein system to understand how the unfolded states of globular proteins drive …

We investigated how mitochondrial membrane proteins remain soluble in the cytosol until
their delivery to mitochondria or degradation at the proteasome. We show that Ubiquilin …

The throughput of cell mechanical characterization has recently approached that of
conventional flow cytometers. However, this very sensitive, label-free approach still lacks the …

We present a fluorogenic method to visualize misfolding and aggregation of a specific
protein-of-interest in live cells using structurally modulated fluorescent protein …

When proteostasis becomes unbalanced, unfolded proteins can accumulate and aggregate.
Here we report that the dye, tetraphenylethene maleimide (TPE-MI) can be used to measure …

Conspectus Protein aggregation is a biological phenomenon in which aberrantly processed
or mutant proteins misfold and assemble into a variety of insoluble aggregates. Decades of …