Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli

A De Marco - Microbial cell factories, 2009 - Springer
Bacteria are simple and cost effective hosts for producing recombinant proteins. However,
their physiological features may limit their use for obtaining in native form proteins of some …

Display technologies: application for the discovery of drug and gene delivery agents

A Sergeeva, MG Kolonin, JJ Molldrem… - Advanced drug delivery …, 2006 - Elsevier
Recognition of molecular diversity of cell surface proteomes in disease is essential for the
development of targeted therapies. Progress in targeted therapeutics requires establishing …

[HTML][HTML] Proteome-wide epitope map** of antibodies using ultra-dense peptide arrays

B Forsström, BB Axnäs, KP Stengele, J Bühler… - Molecular & Cellular …, 2014 - Elsevier
Antibodies are of importance for the field of proteomics, both as reagents for imaging cells,
tissues, and organs and as capturing agents for affinity enrichment in mass-spectrometry …

Antibody-based proteomics: fast-tracking molecular diagnostics in oncology

DJ Brennan, DP O'connor, E Rexhepaj… - Nature Reviews …, 2010 - nature.com
The effective implementation of personalized cancer therapeutic regimens depends on the
successful identification and translation of informative biomarkers to aid clinical decision …

An integrated microfluidic system for reaction, high-sensitivity detection, and sorting of fluorescent cells and particles

PS Dittrich, P Schwille - Analytical chemistry, 2003 - ACS Publications
Presented is a novel approach for an integrated micro total analysis system (μTAS) based
on a microfluidic on-chip device that supports ultrasensitive confocal detection of fluorescent …

[HTML][HTML] Type V secretion: from biogenesis to biotechnology

P Van Ulsen, S ur Rahman, WSP Jong… - … et Biophysica Acta (BBA …, 2014 - Elsevier
The two membranes of Gram-negative bacteria contain protein machines that have a
general function in their assembly. To interact with the extra-cellular milieu, Gram-negatives …

Directed evolution: tailoring biocatalysts for industrial applications

A Kumar, S Singh - Critical reviews in biotechnology, 2013 - Taylor & Francis
Current challenges and promises of white biotechnology encourage protein engineers to
use a directed evolution approach to generate novel and useful biocatalysts for various sets …

Marker vaccine strategies and candidate CSFV marker vaccines

XN Dong, YH Chen - Vaccine, 2007 - Elsevier
Classical swine fever (CSF) is an economically important highly contagious disease of
swine worldwide. Classical swine fever virus (CSFV) is its etiological agent, and the only …

Fine epitope map** of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display

G Chao, JR Cochran, KD Wittrup - Journal of molecular biology, 2004 - Elsevier
Fine epitope map** of therapeutically relevant monoclonal antibodies (mAbs) specific for
the epidermal growth factor receptor (EGFR) was accomplished through random …

Cell‐surface display of heterologous proteins: From high‐throughput screening to environmental applications

W Chen, G Georgiou - Biotechnology and bioengineering, 2002 - Wiley Online Library
A variety of expression systems for the display of either short peptides or fully folded proteins
on E. coli and, to a lesser extent, on Gram‐positive bacteria have been developed. The …