Droplet microfluidics for microbiology: techniques, applications and challenges

TS Kaminski, O Scheler, P Garstecki - Lab on a Chip, 2016 - pubs.rsc.org
Droplet microfluidics has rapidly emerged as one of the key technologies opening up new
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …

From axenic to mixed cultures: technological advances accelerating a paradigm shift in microbiology

C Nai, V Meyer - Trends in microbiology, 2018 - cell.com
Since the onset of microbiology in the late 19th century, scientists have been growing
microorganisms almost exclusively as pure cultures, resulting in a limited and biased view of …

Interindividual variation in dietary carbohydrate metabolism by gut bacteria revealed with droplet microfluidic culture

MM Villa, RJ Bloom, JD Silverman, HK Durand… - Msystems, 2020 - Am Soc Microbiol
Culture and screening of gut bacteria enable testing of microbial function and therapeutic
potential. However, the diversity of human gut microbial communities (microbiota) impedes …

Label-free counting of Escherichia coli cells in nanoliter droplets using 3D printed microfluidic devices with integrated contactless conductivity detection

LC Duarte, F Figueredo, LEB Ribeiro, E Cortón… - Analytica chimica …, 2019 - Elsevier
This study describes for the first time the development of 3D printed microfluidic devices with
integrated electrodes for label-free counting of E. coli cells incorporated inside droplets …

Optimized droplet digital CFU assay (ddCFU) provides precise quantification of bacteria over a dynamic range of 6logs and beyond

O Scheler, N Pacocha, PR Debski, A Ruszczak… - Lab on a Chip, 2017 - pubs.rsc.org
Standard digital assays need a large number of compartments for precise quantification of a
sample over a broad dynamic range. We address this issue with an optimized droplet digital …

Go with the flow or solitary confinement: a look inside the single-cell toolbox for isolation of rare and uncultured microbes

GRB Huys, J Raes - Current opinion in microbiology, 2018 - Elsevier
With the vast majority of the microbial world still considered unculturable or undiscovered,
microbiologists not only require more fundamental insights concerning microbial growth …

Passive microinjection within high-throughput microfluidics for controlled actuation of droplets and cells

M Azarmanesh, M Dejam, P Azizian, G Yesiloz… - Scientific reports, 2019 - nature.com
Microinjection is an effective actuation technique used for precise delivery of molecules and
cells into droplets or controlled delivery of genes, molecules, proteins, and viruses into …

Droplet microfluidics for microbial biotechnology

S Hengoju, M Tovar, DDKW Man, S Buchheim… - Microfluidics in …, 2020 - Springer
Droplet microfluidics has recently evolved as a prominent platform for high-throughput
experimentation for various research fields including microbiology. Key features of droplet …

Tracking the stochastic growth of bacterial populations in microfluidic droplets

D Taylor, N Verdon, P Lomax, RJ Allen… - Physical biology, 2022 - iopscience.iop.org
Bacterial growth in microfluidic droplets is relevant in biotechnology, in microbial ecology,
and in understanding stochastic population dynamics in small populations. However, it has …

Quantitative and analytical tools to analyze the spatiotemporal population dynamics of microbial consortia

Y Liu, P Xu - Current Opinion in Biotechnology, 2022 - Elsevier
Highlights•Reductionism and holism are complementary tools to understand microbial
community.•Spatiotemporal tools and math models to study microbial consortia were …