Droplet microfluidics for microbiology: techniques, applications and challenges
Droplet microfluidics has rapidly emerged as one of the key technologies opening up new
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …
From axenic to mixed cultures: technological advances accelerating a paradigm shift in microbiology
C Nai, V Meyer - Trends in microbiology, 2018 - cell.com
Since the onset of microbiology in the late 19th century, scientists have been growing
microorganisms almost exclusively as pure cultures, resulting in a limited and biased view of …
microorganisms almost exclusively as pure cultures, resulting in a limited and biased view of …
Interindividual variation in dietary carbohydrate metabolism by gut bacteria revealed with droplet microfluidic culture
Culture and screening of gut bacteria enable testing of microbial function and therapeutic
potential. However, the diversity of human gut microbial communities (microbiota) impedes …
potential. However, the diversity of human gut microbial communities (microbiota) impedes …
Label-free counting of Escherichia coli cells in nanoliter droplets using 3D printed microfluidic devices with integrated contactless conductivity detection
This study describes for the first time the development of 3D printed microfluidic devices with
integrated electrodes for label-free counting of E. coli cells incorporated inside droplets …
integrated electrodes for label-free counting of E. coli cells incorporated inside droplets …
Optimized droplet digital CFU assay (ddCFU) provides precise quantification of bacteria over a dynamic range of 6logs and beyond
O Scheler, N Pacocha, PR Debski, A Ruszczak… - Lab on a Chip, 2017 - pubs.rsc.org
Standard digital assays need a large number of compartments for precise quantification of a
sample over a broad dynamic range. We address this issue with an optimized droplet digital …
sample over a broad dynamic range. We address this issue with an optimized droplet digital …
Go with the flow or solitary confinement: a look inside the single-cell toolbox for isolation of rare and uncultured microbes
GRB Huys, J Raes - Current opinion in microbiology, 2018 - Elsevier
With the vast majority of the microbial world still considered unculturable or undiscovered,
microbiologists not only require more fundamental insights concerning microbial growth …
microbiologists not only require more fundamental insights concerning microbial growth …
Passive microinjection within high-throughput microfluidics for controlled actuation of droplets and cells
Microinjection is an effective actuation technique used for precise delivery of molecules and
cells into droplets or controlled delivery of genes, molecules, proteins, and viruses into …
cells into droplets or controlled delivery of genes, molecules, proteins, and viruses into …
Droplet microfluidics for microbial biotechnology
S Hengoju, M Tovar, DDKW Man, S Buchheim… - Microfluidics in …, 2020 - Springer
Droplet microfluidics has recently evolved as a prominent platform for high-throughput
experimentation for various research fields including microbiology. Key features of droplet …
experimentation for various research fields including microbiology. Key features of droplet …
Tracking the stochastic growth of bacterial populations in microfluidic droplets
D Taylor, N Verdon, P Lomax, RJ Allen… - Physical biology, 2022 - iopscience.iop.org
Bacterial growth in microfluidic droplets is relevant in biotechnology, in microbial ecology,
and in understanding stochastic population dynamics in small populations. However, it has …
and in understanding stochastic population dynamics in small populations. However, it has …
Quantitative and analytical tools to analyze the spatiotemporal population dynamics of microbial consortia
Y Liu, P Xu - Current Opinion in Biotechnology, 2022 - Elsevier
Highlights•Reductionism and holism are complementary tools to understand microbial
community.•Spatiotemporal tools and math models to study microbial consortia were …
community.•Spatiotemporal tools and math models to study microbial consortia were …