Manufacturing of non-viral protein nanocages for biotechnological and biomedical applications

J João, DMF Prazeres - Frontiers in Bioengineering and …, 2023 - frontiersin.org
Protein nanocages are highly ordered nanometer scale architectures, which are typically
formed by homo-or hetero-self-assembly of multiple monomers into symmetric structures of …

[HTML][HTML] Development of a Two-Component Nanoparticle Vaccine Displaying an HIV-1 Envelope Glycoprotein that Elicits Tier 2 Neutralising Antibodies

K Malebo, J Woodward, P **mba, Q Mkhize, S Cingo… - Vaccines, 2024 - mdpi.com
Despite treatment and other interventions, an effective prophylactic HIV vaccine is still an
essential goal in the control of HIV. Inducing robust and long-lasting antibody responses is …

Mitigating the Blurring Effect of CryoEM Averaging on a Flexible and Highly Symmetric Protein Complex through Sub-Particle Reconstruction

DS Suder, S Gonen - International Journal of Molecular Sciences, 2024 - mdpi.com
Many macromolecules are inherently flexible as a feature of their structure and function.
During single-particle CryoEM processing, flexible protein regions can be detrimental to …

[HTML][HTML] Quantitative Comparison of Monomeric StayGold Variants Using Protein Nanocages in Living Cells

G Viola, KA Jacobs, J Lemière, ML Kutys… - bioRxiv, 2024 - pmc.ncbi.nlm.nih.gov
To standardize comparison of fluorescent proteins and independently determine which
monomeric StayGold variant is best for live microscopy, we analyzed fluorescent protein …