New insights into protein–protein interaction modulators in drug discovery and therapeutic advance

H Nada, Y Choi, S Kim, KS Jeong… - … and Targeted Therapy, 2024 - nature.com
Protein-protein interactions (PPIs) are fundamental to cellular signaling and transduction
which marks them as attractive therapeutic drug development targets. What were once …

Cancer and neurodevelopmental disorders: multi-scale reasoning and computational guide

R Nussinov, BR Yavuz, HC Demirel, MK Arici… - Frontiers in Cell and …, 2024 - frontiersin.org
The connection and causality between cancer and neurodevelopmental disorders have
been puzzling. How can the same cellular pathways, proteins, and mutations lead to …

Tumor-derived RHOA mutants interact with effectors in the GDP-bound state

Y Lin, TA Ramelot, S Senyuz, A Gursoy, H Jang… - Nature …, 2024 - nature.com
RHOA mutations are found at diverse residues in various cancer types, implying mutation-
and cell-specific mechanisms of tumorigenesis. Here, we focus on the underlying …

Hydrogen/deuterium exchange mass spectrometry: fundamentals, limitations, and opportunities

L Konermann, PM Scrosati - Molecular & Cellular Proteomics, 2024 - mcponline.org
Hydrogen/deuterium exchange mass spectrometry (HDX-MS) probes dynamic motions of
proteins by monitoring the kinetics of backbone amide deuteration. Dynamic regions exhibit …

The value of protein allostery in rational anticancer drug design: an update

R Nussinov, H Jang - Expert Opinion on Drug Discovery, 2024 - Taylor & Francis
Introduction Allosteric drugs are advantageous. However, they still face hurdles, including
identification of allosteric sites that will effectively alter the active site. Current strategies …

[HTML][HTML] Single cell spatial biology over developmental time can decipher pediatric brain pathologies

R Nussinov, BR Yavuz, H Jang - Neurobiology of Disease, 2024 - Elsevier
Pediatric low grade brain tumors and neurodevelopmental disorders share proteins,
signaling pathways, and networks. They also share germline mutations and an impaired …

[HTML][HTML] Cell phenotypes can be predicted from propensities of protein conformations

R Nussinov, Y Liu, W Zhang, H Jang - Current Opinion in Structural Biology, 2023 - Elsevier
Proteins exist as dynamic conformational ensembles. Here we suggest that the propensities
of the conformations can be predictors of cell function. The conformational states that the …

The allosteric mechanism of mTOR activation can inform bitopic inhibitor optimization

Y Liu, M Zhang, H Jang, R Nussinov - Chemical Science, 2024 - pubs.rsc.org
mTOR serine/threonine kinase is a cornerstone in the PI3K/AKT/mTOR pathway. Yet, the
detailed mechanism of activation of its catalytic core is still unresolved, likely due to mTOR …

CDK2 and CDK4: Cell Cycle Functions Evolve Distinct, Catalysis-Competent Conformations, Offering Drug Targets

W Zhang, Y Liu, H Jang, R Nussinov - JACS Au, 2024 - ACS Publications
Cyclin-dependent kinases (CDKs), particularly CDK4 and CDK2, are crucial for cell cycle
progression from the Gap 1 (G1) to the Synthesis (S) phase by phosphorylating targets such …

Capturing Autoinhibited PDK1 Reveals the Linker's Regulatory Role, Informing Innovative Inhibitor Design

L Xu, H Jang, R Nussinov - Journal of Chemical Information and …, 2024 - ACS Publications
PDK1 is crucial for PI3K/AKT/mTOR and Ras/MAPK cancer signaling. It phosphorylates AKT
in a PIP3-dependent but S6K, SGK, and RSK kinases in a PIP3-independent manner. Unlike …