Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy

M Chehelgerdi, M Chehelgerdi… - Molecular cancer, 2024 - Springer
The CRISPR system is a revolutionary genome editing tool that has the potential to
revolutionize the field of cancer research and therapy. The ability to precisely target and edit …

[HTML][HTML] Review of COVID-19 testing and diagnostic methods

O Filchakova, D Dossym, A Ilyas, T Kuanysheva… - Talanta, 2022 - Elsevier
More than six billion tests for COVID-19 has been already performed in the world. The
testing for SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) virus and …

Uncovering the functional diversity of rare CRISPR-Cas systems with deep terascale clustering

H Altae-Tran, S Kannan, AJ Suberski, KS Mears… - Science, 2023 - science.org
Microbial systems underpin many biotechnologies, including CRISPR, but the exponential
growth of sequence databases makes it difficult to find previously unidentified systems. In …

CRISPR–cas9: A history of its discovery and ethical considerations of its use in genome editing

I Gostimskaya - Biochemistry (Moscow), 2022 - Springer
The development of a method for genome editing based on CRISPR–Cas9 technology was
awarded The Nobel Prize in Chemistry in 2020, less than a decade after the discovery of all …

The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases

H Altae-Tran, S Kannan, FE Demircioglu, R Oshiro… - Science, 2021 - science.org
IscB proteins are putative nucleases encoded in a distinct family of IS200/IS605 transposons
and are likely ancestors of the RNA-guided endonuclease Cas9, but the functions of IscB …

[HTML][HTML] Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

P Fozouni, S Son, MD de León Derby, GJ Knott… - Cell, 2021 - cell.com
The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an
ongoing global pandemic due in part to the challenge of identifying symptomatic …

Structural biology of CRISPR–Cas immunity and genome editing enzymes

JY Wang, P Pausch, JA Doudna - Nature Reviews Microbiology, 2022 - nature.com
CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …

Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

AV Anzalone, LW Koblan, DR Liu - Nature biotechnology, 2020 - nature.com
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …

Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease

T Karvelis, G Druteika, G Bigelyte, K Budre… - Nature, 2021 - nature.com
Transposition has a key role in resha** genomes of all living organisms. Insertion
sequences of IS200/IS605 and IS607 families are among the simplest mobile genetic …

COVID-19: Transmission, prevention, and potential therapeutic opportunities

M Lotfi, MR Hamblin, N Rezaei - Clinica chimica acta, 2020 - Elsevier
The novel coronavirus disease (COVID-19) pandemic, caused by severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2), remains a global challenge. Despite intense …