Perusal of food allergens analysis by mass spectrometry-based proteomics

V Marzano, B Tilocca, AG Fiocchi, P Vernocchi… - Journal of …, 2020‏ - Elsevier
Food allergy is the disease where the immune system is elicited by antigens in food.
Although innocuous for immune-tolerant individuals, an ever-growing number of food …

High-quality and robust protein quantification in large clinical/pharmaceutical cohorts with IonStar proteomics investigation

S Shen, X Wang, X Zhu, S Rasam, M Ma, S Huo… - Nature protocols, 2023‏ - nature.com
Robust, reliable quantification of large sample cohorts is often essential for meaningful
clinical or pharmaceutical proteomics investigations, but it is technically challenging. When …

Combined proximity labeling and affinity purification− mass spectrometry workflow for map** and visualizing protein interaction networks

X Liu, K Salokas, RG Weldatsadik, L Gawriyski… - Nature protocols, 2020‏ - nature.com
Affinity purification coupled with mass spectrometry (AP–MS) and proximity-dependent
biotinylation identification (BioID) methods have made substantial contributions to …

Non-Thermal Processing Technologies for Allergen Control in Alternative Protein Sources for Food Industry Applications

G Dong, LM Hinds, AB Soro, Z Hu, DW Sun… - Food Engineering …, 2024‏ - Springer
Sustainable food practices within the food industry are pertinent to allow efficient food supply
while not negatively impacting the environment. Alternative proteins have gained the …

mokapot: Fast and flexible semisupervised learning for peptide detection

WE Fondrie, WS Noble - Journal of Proteome Research, 2021‏ - ACS Publications
Proteomics studies rely on the accurate assignment of peptides to the acquired tandem
mass spectra—a task where machine learning algorithms have proven invaluable. We …

[HTML][HTML] A systematic evaluation of yeast sample preparation protocols for spectral identifications, proteome coverage and post-isolation modifications

M den Ridder, E Knibbe, W van den Brandeler… - Journal of …, 2022‏ - Elsevier
The importance of obtaining comprehensive and accurate information from cellular
proteomics experiments asks for a systematic investigation of sample preparation protocols …

Using the entrapment sequence method as a standard to evaluate key steps of proteomics data analysis process

X Feng, L Li, J Zhang, Y Zhu, C Chang, K Shu, J Ma - BMC genomics, 2017‏ - Springer
Background The mass spectrometry based technical pipeline has provided a high-
throughput, high-sensitivity and high-resolution platform for post-genomic biology. Varied …

Machine learning strategy that leverages large data sets to boost statistical power in small-scale experiments

WE Fondrie, WS Noble - Journal of Proteome Research, 2020‏ - ACS Publications
Machine learning methods have proven invaluable for increasing the sensitivity of peptide
detection in proteomics experiments. Most modern tools, such as Percolator and …

Performance investigation of proteomic identification by HCD/CID fragmentations in combination with high/low-resolution detectors on a tribrid, high-field orbitrap …

C Tu, J Li, S Shen, Q Sheng, Y Shyr, J Qu - PloS one, 2016‏ - journals.plos.org
The recently-introduced Orbitrap Fusion mass spectrometry permits various types of MS2
acquisition methods. To date, these different MS2 strategies and the optimal data …

AIomics: exploring more of the proteome using mass spectral libraries extended by artificial intelligence

LY Geer, J Lapin, DJ Slotta, TD Mak… - Journal of proteome …, 2023‏ - ACS Publications
The unbounded permutations of biological molecules, including proteins and their
constituent peptides, present a dilemma in identifying the components of complex …