Raman microspectroscopy for microbiology

KS Lee, Z Landry, FC Pereira, M Wagner… - Nature Reviews …, 2021 - nature.com
Raman microspectroscopy offers microbiologists a rapid and non-destructive technique to
assess the chemical composition of individual live microorganisms in near real time. In this …

Recent developments in Raman spectral analysis of microbial single cells: Techniques and applications

H Jayan, H Pu, DW Sun - Critical Reviews in Food Science and …, 2022 - Taylor & Francis
The conventional microbial cell analyses are mostly population-averaged methods that
conceal the characteristics of single-cell in the community. Single-cell analysis can provide …

Raman spectroscopy and imaging in bioanalytics

D Cialla-May, C Krafft, P Rösch… - Analytical …, 2021 - ACS Publications
Since the discovery of the inelastic scattering of light, ie, the so-called Raman effect, 1
Raman spectroscopy has become an attractive tool in a high number of research fields …

Metabolic-Activity-Based Assessment of Antimicrobial Effects by D2O-Labeled Single-Cell Raman Microspectroscopy

Y Tao, Y Wang, S Huang, P Zhu, WE Huang… - Analytical …, 2017 - ACS Publications
To combat the spread of antibiotic resistance, methods that quantitatively assess the
metabolism-inhibiting effects of drugs in a rapid and culture-independent manner are …

Raman-activated droplet sorting (RADS) for label-free high-throughput screening of microalgal single-cells

X Wang, L Ren, Y Su, Y Ji, Y Liu, C Li, X Li… - Analytical …, 2017 - ACS Publications
Raman-activated cell sorting (RACS) has attracted increasing interest, yet throughput
remains one major factor limiting its broader application. Here we present an integrated …

Robust spontaneous Raman flow cytometry for single‐cell metabolic phenome profiling via pDEP‐DLD‐RFC

X Wang, L Ren, Z Diao, Y He, J Zhang, M Liu… - Advanced …, 2023 - Wiley Online Library
A full‐spectrum spontaneous single‐cell Raman spectrum (fs‐SCRS) captures the metabolic
phenome for a given cellular state of the cell in a label‐free, landscape‐like manner. Herein …

Positive dielectrophoresis–based Raman-activated droplet sorting for culture-free and label-free screening of enzyme function in vivo

X Wang, Y **n, L Ren, Z Sun, P Zhu, Y Ji, C Li, J Xu… - Science …, 2020 - science.org
The potential of Raman-activated cell sorting (RACS) is inherently limited by conflicting
demands for signal quality and sorting throughput. Here, we present positive …

Development of a fast Raman-assisted antibiotic susceptibility test (FRAST) for the antibiotic resistance analysis of clinical urine and blood samples

X Yi, Y Song, X Xu, D Peng, J Wang, X Qie… - Analytical …, 2021 - ACS Publications
Human health is at great risk due to the spreading of antimicrobial resistance (AMR). The
lengthy procedure of conventional antimicrobial susceptibility testing (AST) usually requires …

Antibiotic resistance diagnosis in ESKAPE pathogens—a review on proteomic perspective

S Kalpana, WY Lin, YC Wang, Y Fu, A Lakshmi… - Diagnostics, 2023 - mdpi.com
Antibiotic resistance has emerged as an imminent pandemic. Rapid diagnostic assays
distinguish bacterial infections from other diseases and aid antimicrobial stewardship …

Advances in single cell Raman spectroscopy technologies for biological and environmental applications

D Wang, P He, Z Wang, G Li, N Majed, AZ Gu - Current opinion in …, 2020 - Elsevier
The increasing sophistication of single cell Raman spectroscopy (SCRS) via its integrations
with other advanced analytical techniques and modern data analytics, enable …