Next-generation sequencing technologies: An overview

T Hu, N Chitnis, D Monos, A Dinh - Human Immunology, 2021 - Elsevier
Since the days of Sanger sequencing, next-generation sequencing technologies have
significantly evolved to provide increased data output, efficiencies, and applications. These …

B cells and tertiary lymphoid structures promote immunotherapy response

BA Helmink, SM Reddy, J Gao, S Zhang, R Basar… - Nature, 2020 - nature.com
Abstract Treatment with immune checkpoint blockade (ICB) has revolutionized cancer
therapy. Until now, predictive biomarkers,,,,,,,,–and strategies to augment clinical response …

[HTML][HTML] gprofiler2--an R package for gene list functional enrichment analysis and namespace conversion toolset g: Profiler

L Kolberg, U Raudvere, I Kuzmin, J Vilo… - …, 2020 - ncbi.nlm.nih.gov
Abstract g: Profiler (https://biit. cs. ut. ee/gprofiler) is a widely used gene list functional
profiling and namespace conversion toolset that has been contributing to reproducible …

Regulatory role of microRNAs (miRNAs) in the recent development of abiotic stress tolerance of plants

Y Begum - Gene, 2022 - Elsevier
MicroRNAs (miRNAs) are a distinct groups of single-stranded non-coding, tiny regulatory
RNAs approximately 20–24 nucleotides in length. miRNAs negatively influence gene …

[HTML][HTML] A step-by-step workflow for low-level analysis of single-cell RNA-seq data with Bioconductor

ATL Lun, DJ McCarthy, JC Marioni - F1000Research, 2016 - ncbi.nlm.nih.gov
Single-cell RNA sequencing (scRNA-seq) is widely used to profile the transcriptome of
individual cells. This provides biological resolution that cannot be matched by bulk RNA …

Distinct immune cell populations define response to anti-PD-1 monotherapy and anti-PD-1/anti-CTLA-4 combined therapy

TN Gide, C Quek, AM Menzies, AT Tasker, P Shang… - Cancer cell, 2019 - cell.com
Cancer immunotherapies provide survival benefits in responding patients, but many patients
fail to respond. Identifying the biology of treatment response and resistance are a priority to …

[HTML][HTML] From reads to genes to pathways: differential expression analysis of RNA-Seq experiments using Rsubread and the edgeR quasi-likelihood pipeline

Y Chen, ATL Lun, GK Smyth - F1000Research, 2016 - ncbi.nlm.nih.gov
In recent years, RNA sequencing (RNA-seq) has become a very widely used technology for
profiling gene expression. One of the most common aims of RNA-seq profiling is to identify …

Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4/NFκB/NLRP3 signaling …

L Luo, M Liu, Y Fan, J Zhang, L Liu, Y Li… - Journal of …, 2022 - Springer
Background Neuronal pyroptosis and neuroinflammation with excess microglial activation
are widely involved in the early pathological process of ischemic stroke. Repetitive …

Ribosome footprint profiling of translation throughout the genome

NT Ingolia - Cell, 2016 - cell.com
Ribosome profiling has emerged as a technique for measuring translation comprehensively
and quantitatively by deep sequencing of ribosome-protected mRNA fragments. By …

[HTML][HTML] Large-scale topological changes restrain malignant progression in colorectal cancer

SE Johnstone, A Reyes, Y Qi, C Adriaens, E Hegazi… - Cell, 2020 - cell.com
Widespread changes to DNA methylation and chromatin are well documented in cancer, but
the fate of higher-order chromosomal structure remains obscure. Here we integrated …