Cryo-electron tomography on focused ion beam lamellae transforms structural cell biology
Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …
of isolated macromolecules to near-atomic resolution. However, these data do not provide …
[HTML][HTML] Structure and mechanism of the RNA polymerase II transcription machinery
AC Schier, DJ Taatjes - Genes & development, 2020 - genesdev.cshlp.org
Abstract RNA polymerase II (Pol II) transcribes all protein-coding genes and many
noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it …
noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it …
New tools for automated cryo-EM single-particle analysis in RELION-4.0
D Kimanius, L Dong, G Sharov, T Nakane… - Biochemical …, 2021 - portlandpress.com
We describe new tools for the processing of electron cryo-microscopy (cryo-EM) images in
the fourth major release of the RELION software. In particular, we introduce VDAM, a …
the fourth major release of the RELION software. In particular, we introduce VDAM, a …
Structure-based classification of tauopathies
The ordered assembly of tau protein into filaments characterizes several neurodegenerative
diseases, which are called tauopathies. It was previously reported that, by cryo-electron …
diseases, which are called tauopathies. It was previously reported that, by cryo-electron …
Receptor binding and priming of the spike protein of SARS-CoV-2 for membrane fusion
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is initiated by
virus binding to the ACE2 cell-surface receptors,,–, followed by fusion of the virus and cell …
virus binding to the ACE2 cell-surface receptors,,–, followed by fusion of the virus and cell …
Distinct conformational states of SARS-CoV-2 spike protein
Intervention strategies are urgently needed to control the severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) pandemic. The trimeric viral spike (S) protein catalyzes fusion …
coronavirus 2 (SARS-CoV-2) pandemic. The trimeric viral spike (S) protein catalyzes fusion …
Membrane fusion and immune evasion by the spike protein of SARS-CoV-2 Delta variant
The Delta variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has
outcompeted previously prevalent variants and become a dominant strain worldwide. We …
outcompeted previously prevalent variants and become a dominant strain worldwide. We …
Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2
The SARS-CoV-2 virus is more transmissible than previous coronaviruses and causes a
more serious illness than influenza. The SARS-CoV-2 receptor binding domain (RBD) of the …
more serious illness than influenza. The SARS-CoV-2 receptor binding domain (RBD) of the …
DeepEMhancer: a deep learning solution for cryo-EM volume post-processing
Cryo-EM maps are valuable sources of information for protein structure modeling. However,
due to the loss of contrast at high frequencies, they generally need to be post-processed to …
due to the loss of contrast at high frequencies, they generally need to be post-processed to …
SARS-CoV-2 and bat RaTG13 spike glycoprotein structures inform on virus evolution and furin-cleavage effects
SARS-CoV-2 is thought to have emerged from bats, possibly via a secondary host. Here, we
investigate the relationship of spike (S) glycoprotein from SARS-CoV-2 with the S protein of …
investigate the relationship of spike (S) glycoprotein from SARS-CoV-2 with the S protein of …