Molecular rotors: fluorescent sensors for microviscosity and conformation of biomolecules

M Paez‐Perez, MK Kuimova - Angewandte Chemie, 2024 - Wiley Online Library
The viscosity and crowding of biological environment are considered vital for the correct
cellular function, and alterations in these parameters are known to underly a number of …

[HTML][HTML] Liquid-liquid phase separation as a common organizing principle of intracellular space and biomembranes providing dynamic adaptive responses

SV Nesterov, NS Ilyinsky, VN Uversky - Biochimica et Biophysica Acta (BBA …, 2021 - Elsevier
This work is devoted to the phenomenon of liquid-liquid phase separation (LLPS), which has
come to be recognized as fundamental organizing principle of living cells. We distinguish …

Small-molecule fluorescent probes for plasma membrane staining: Design, mechanisms and biological applications

L Yang, Q Chen, Z Wang, H Zhang, H Sun - Coordination Chemistry …, 2023 - Elsevier
Plasma membrane (PM), a phospholipid bilayer that separates the interior of cell from the
extracellular fluid, is closely involved in various cellular activities. However, monitoring …

Cell surface-localized imaging and sensing

HR Jia, YX Zhu, QY Duan, FG Wu - Chemical Society Reviews, 2021 - pubs.rsc.org
Systematically dissecting the molecular basis of the cell surface as well as its related
biological activities is considered as one of the most cutting-edge fields in fundamental …

Designing a green-emitting viscosity-sensitive 4, 4-difluoro-4-bora-3a, 4a-diaza-s-indacene (BODIPY) probe for plasma membrane viscosity imaging

A Polita, M Stancikaitė, R Žvirblis, K Maleckaitė… - RSC …, 2023 - pubs.rsc.org
Viscosity is a key characteristic of lipid membranes–it governs the passive diffusion of
solutes and affects the lipid raft formation and membrane fluidity. Precise determination of …

Exploring viscosity, polarity and temperature sensitivity of BODIPY-based molecular rotors

A Vyšniauskas, I López-Duarte, N Duchemin… - Physical Chemistry …, 2017 - pubs.rsc.org
Microviscosity is a key parameter controlling the rate of diffusion and reactions on the
microscale. One of the most convenient tools for measuring microviscosity is by fluorescent …

Directly imaging emergence of phase separation in peroxidized lipid membranes

M Paez-Perez, A Vyšniauskas, I López-Duarte… - Communications …, 2023 - nature.com
Lipid peroxidation is a process which is key in cell signaling and disease, it is exploited in
cancer therapy in the form of photodynamic therapy. The appearance of hydrophilic moieties …

Cyclopropyl substituents transform the viscosity-sensitive BODIPY molecular rotor into a temperature sensor

A Vyšniauskas, B Cornell, PS Sherin… - ACS …, 2021 - ACS Publications
A quantitative fluorescent probe that responds to changes in temperature is highly desirable
for studies of biological environments, particularly in cellulo. Here, we report new cell …

The role of plasma membrane viscosity in the response and resistance of cancer cells to oxaliplatin

L Shimolina, A Gulin, N Ignatova, I Druzhkova… - Cancers, 2021 - mdpi.com
Simple Summary Understanding the role of the plasma membrane in the responses of
cancer cells to chemotherapy is important because the cell membrane is directly involved in …

Endomembrane tension and trafficking

A Saric, SA Freeman - Frontiers in Cell and Developmental Biology, 2021 - frontiersin.org
Eukaryotic cells employ diverse uptake mechanisms depending on their specialized
functions. While such mechanisms vary widely in their defining criteria: scale, molecular …