For a long time, cysteine cathepsins were considered primarily as proteases crucial for
nonspecific bulk proteolysis in the endolysosomal system. However, this view has …

Despite advances in genetic and proteomic techniques, a complete portrait of the proteome
and its complement of dynamic interactions and modifications remains a lofty, and as of yet …

Activity-based protein profiling (ABPP) is a technique that uses highly selective active-site
targeted chemical probes to label and monitor the state of proteins. ABPP integrates the …

Cysteine can be specifically functionalized by a myriad of acid-base conjugation strategies
for applications ranging from probing protein function to antibody-drug conjugates and …

In contrast to the traditional mechanism of drug action that relies on the reversible,
noncovalent interaction of a ligand with its biological target, a targeted covalent inhibitor …

Small molecules can serve as leads for new therapeutics as well as powerful tools to
investigate biological processes. Understanding the interactions of these molecules …

Conspectus Metal ions can be beneficial or toxic depending on their identity, oxidation state,
and concentration. Therefore, the ability to detect and quantify different types of metal ions …

Fluorescent tools have revolutionized our ability to probe biological dynamics, particularly at
the cellular level. Fluorescent sensors have been developed on several platforms, utilizing …

Conspectus One of the biggest bottlenecks in modern drug discovery efforts is in tackling the
undruggable proteome. Currently, over 85% of the proteome is still considered undruggable …

This review presents some of the hottest topics in biotechnological applications: proteases in
biocatalysis. Obviously, one of the most relevant areas of application is in the hydrolysis of …