RING finger domain and RING finger-like ubiquitin ligases (E3s), such as U-box proteins,
constitute the vast majority of known E3s. RING-type E3s function together with ubiquitin …

3D cryo-electron microscopy (cryo-EM) is an expanding structural biology technique that has
recently undergone a quantum leap progression in its achievable resolution and its …

Ubiquitin modification is mediated by a large family of specificity determining ubiquitin E3
ligases. To facilitate ubiquitin transfer, RING E3 ligases bind both substrate and a ubiquitin …

Analyses of protein complexes are facilitated by methods that enable the generation of
recombinant complexes via coexpression of their subunits from multigene DNA constructs …

One does not often look to analytic cubism for insights into the control of the cell cycle, but
Pablo Picasso beautifully encapsulated the fundamentals when he said that “every act of …

The appropriate timing of events that lead to chromosome segregation during mitosis and
cytokinesis is essential to prevent aneuploidy, and defects in these processes can contribute …

Both ubiquitination and SUMOylation are dynamic post-translational modifications that
regulate thousands of target proteins to control virtually every cellular process …

In mitosis, the spindle assembly checkpoint (SAC) ensures genome stability by delaying
chromosome segregation until all sister chromatids have achieved bipolar attachment to the …

Abstract The Anaphase Promoting Complex (APC, also called APC/C) regulates cell cycle
progression by forming two closely related, but functionally distinct E3 ubiquitin ligase sub …

Native mass spectrometry (MS) is aimed at preserving and determining the native structure,
composition, and stoichiometry of biomolecules and their complexes from solution after they …