Organoids are in vitro miniaturized and simplified model systems of organs that have gained
enormous interest for modelling tissue development and disease, and for personalized …

Multicellular spheroids are three dimensional in vitro microscale tissue analogs. The current
article examines the suitability of spheroids as an in vitro platform for testing drug delivery …

The prostate gland consists of basal and luminal cells arranged as pseudostratified
epithelium. In tissue recombination models, only basal cells reconstitute a complete prostate …

This protocol describes a strategy for the generation of 3D prostate organoid cultures from
healthy mouse and human prostate cells (either bulk or FACS-sorted single luminal and …

Recently, many types of in vitro 3‐D culture systems have been developed to recapitulate
the in vivo growth conditions of cancer. The cancer 3‐D culture methods aim to preserve the …

The past decade has seen an explosion in the field of in vitro disease modelling, in
particular the development of organoids. These self-organizing tissues derived from stem …

In vitro three-dimensional (3D) cultures are emerging as novel systems with which to study
tissue development, organogenesis and stem cell behavior ex vivo. When grown in a 3D …

Background At the surface of body organs, epithelial tissues must withstand harsh external
environments. To do so, they rely heavily upon stem cells to replenish and repair wounds …

The intrinsic ability to exhibit self-organizing morphogenetic properties in ex vivo culture may
represent a general property of tissue stem cells. Here we show that single luminal …

Cancer Stem Cells (CSCs) are a sub-population of cells, identified in most tumors,
responsible for the initiation, recurrence, metastatic potential, and resistance of different …