Single-cell proteomics enabled by next-generation sequencing or mass spectrometry

HM Bennett, W Stephenson, CM Rose, S Darmanis - Nature Methods, 2023‏ - nature.com
In the last decade, single-cell RNA sequencing routinely performed on large numbers of
single cells has greatly advanced our understanding of the underlying heterogeneity of …

Applications of single-cell RNA sequencing in drug discovery and development

B Van de Sande, JS Lee, E Mutasa-Gottgens… - Nature Reviews Drug …, 2023‏ - nature.com
Single-cell technologies, particularly single-cell RNA sequencing (scRNA-seq) methods,
together with associated computational tools and the growing availability of public data …

Single-cell and spatial transcriptomics: deciphering brain complexity in health and disease

M Piwecka, N Rajewsky, A Rybak-Wolf - Nature Reviews Neurology, 2023‏ - nature.com
In the past decade, single-cell technologies have proliferated and improved from their
technically challenging beginnings to become common laboratory methods capable of …

Single‐cell RNA sequencing technologies and applications: A brief overview

D Jovic, X Liang, H Zeng, L Lin, F Xu… - Clinical and …, 2022‏ - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

[HTML][HTML] Embryo model completes gastrulation to neurulation and organogenesis

G Amadei, CE Handford, C Qiu, J De Jonghe… - Nature, 2022‏ - nature.com
Embryonic stem (ES) cells can undergo many aspects of mammalian embryogenesis in vitro
1, 2, 3, 4, 5, but their developmental potential is substantially extended by interactions with …

Advances in single-cell RNA sequencing and its applications in cancer research

D Huang, N Ma, X Li, Y Gou, Y Duan, B Liu… - Journal of hematology & …, 2023‏ - Springer
Cancers are a group of heterogeneous diseases characterized by the acquisition of
functional capabilities during the transition from a normal to a neoplastic state. Powerful …

Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly

H Li, J Janssens, M De Waegeneer, SS Kolluru… - Science, 2022‏ - science.org
For more than 100 years, the fruit fly Drosophila melanogaster has been one of the most
studied model organisms. Here, we present a single-cell atlas of the adult fly, Tabula …

A human cell atlas of fetal gene expression

J Cao, DR O'day, HA Pliner, PD Kingsley, M Deng… - Science, 2020‏ - science.org
INTRODUCTION A reference atlas of human cell types is a major goal for the field. Here, we
set out to generate single-cell atlases of both gene expression (this study) and chromatin …

GAGE-seq concurrently profiles multiscale 3D genome organization and gene expression in single cells

T Zhou, R Zhang, D Jia, RT Doty, AD Munday, D Gao… - Nature …, 2024‏ - nature.com
The organization of mammalian genomes features a complex, multiscale three-dimensional
(3D) architecture, whose functional significance remains elusive because of limited single …

RNA sequencing: new technologies and applications in cancer research

M Hong, S Tao, L Zhang, LT Diao, X Huang… - Journal of hematology & …, 2020‏ - Springer
Over the past few decades, RNA sequencing has significantly progressed, becoming a
paramount approach for transcriptome profiling. The revolution from bulk RNA sequencing …