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Best practices in metabarcoding of fungi: from experimental design to results
The development of high‐throughput sequencing (HTS) technologies has greatly improved
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …
Mycobiome diversity: high-throughput sequencing and identification of fungi
Fungi are major ecological players in both terrestrial and aquatic environments by cycling
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …
organic matter and channelling nutrients across trophic levels. High-throughput sequencing …
Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?
True fungi (Fungi) and fungus-like organisms (eg Mycetozoa, Oomycota) constitute the
second largest group of organisms based on global richness estimates, with around 3 …
second largest group of organisms based on global richness estimates, with around 3 …
Ranking the biases: The choice of OTUs vs. ASVs in 16S rRNA amplicon data analysis has stronger effects on diversity measures than rarefaction and OTU identity …
Advances in the analysis of amplicon sequence datasets have introduced a methodological
shift in how research teams investigate microbial biodiversity, away from sequence identity …
shift in how research teams investigate microbial biodiversity, away from sequence identity …
[HTML][HTML] Airborne environmental DNA for terrestrial vertebrate community monitoring
Biodiversity monitoring at the community scale is a critical element of assessing and
studying species distributions, ecology, diversity, and movements, and it is key to …
studying species distributions, ecology, diversity, and movements, and it is key to …
High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution
Targeted PCR amplification and high-throughput sequencing (amplicon sequencing) of 16S
rRNA gene fragments is widely used to profile microbial communities. New long-read …
rRNA gene fragments is widely used to profile microbial communities. New long-read …
Diversity and ecology of protists revealed by metabarcoding
Protists are the dominant eukaryotes in the biosphere where they play key functional roles.
While protists have been studied for over a century, it is the high-throughput sequencing of …
While protists have been studied for over a century, it is the high-throughput sequencing of …
[HTML][HTML] ITS alchemy: on the use of ITS as a DNA marker in fungal ecology
H Kauserud - Fungal Ecology, 2023 - Elsevier
High throughput sequencing of PCR amplicons derived from environmental DNA (aka DNA
metabarcoding) has become an integral part of fungal ecology, enabling in-depth …
metabarcoding) has become an integral part of fungal ecology, enabling in-depth …
Bioinformatics matters: The accuracy of plant and soil fungal community data is highly dependent on the metabarcoding pipeline
Fungal communities associated with plants and soil influence plant fitness and ecosystem
functioning. They are frequently studied by metabarcoding approaches targeting the …
functioning. They are frequently studied by metabarcoding approaches targeting the …
Promises and pitfalls of using high‐throughput sequencing for diet analysis
The application of high‐throughput sequencing‐based approaches to DNA extracted from
environmental samples such as gut contents and faeces has become a popular tool for …
environmental samples such as gut contents and faeces has become a popular tool for …