Molecular mechanisms of processive glycoside hydrolases underline catalytic pragmatism

M Hrmova, JG Schwerdt - Biochemical Society Transactions, 2023‏ - portlandpress.com
Processive and distributive catalysis defines the conversion continuum, thus underpinning
the transformation of oligo-and polymeric substrates by enzymes. Distributive catalysis …

ssDNA accessibility of Rad51 is regulated by orchestrating multiple RPA dynamics

J Ding, X Li, J Shen, Y Zhao, S Zhong, L Lai… - Nature …, 2023‏ - nature.com
The eukaryotic single-stranded DNA (ssDNA)-binding protein Replication Protein A (RPA)
plays a crucial role in various DNA metabolic pathways, including DNA replication and …

Artificial Processive Catalytic Systems

RJM Nolte, JAAW Elemans - Chemistry–A European Journal, 2024‏ - Wiley Online Library
Processive catalysts remain attached to a substrate and perform multiple rounds of catalysis.
They are abundant in nature. This review highlights artificial processive catalytic systems …

PLK1 regulates CtIP and DNA2 interplay in long-range DNA end resection

I Ceppi, E Cannavo, H Bret, R Camarillo… - Genes & …, 2023‏ - genesdev.cshlp.org
DNA double-strand break (DSB) repair is initiated by DNA end resection. CtIP acts in short-
range resection to stimulate MRE11–RAD50–NBS1 (MRN) to endonucleolytically cleave …

The MRN complex and topoisomerase IIIa–RMI1/2 synchronize DNA resection motor proteins

MM Soniat, G Nguyen, HC Kuo, IJ Finkelstein - Journal of Biological …, 2023‏ - jbc.org
DNA resection—the nucleolytic processing of broken DNA ends—is the first step of
homologous recombination. Resection is catalyzed by the resectosome, a multienzyme …

Distinct RPA functions promote eukaryotic DNA replication initiation and elongation

AM Pike, CM Friend, SP Bell - Nucleic Acids Research, 2023‏ - academic.oup.com
Replication protein A (RPA) binds single-stranded DNA (ssDNA) and serves critical
functions in eukaryotic DNA replication, the DNA damage response, and DNA repair. During …

The canonical RPA complex interacts with Est3 to regulate yeast telomerase activity

CA Moeller-McCoy, TA Wieser, JW Lubin… - Proceedings of the …, 2025‏ - pnas.org
In most eukaryotic organisms, cells that rely on continuous cell division employ the enzyme
telomerase which replenishes chromosome termini through the addition of telomeric …

Targeting CK2-mediated phosphorylation of p53R2 sensitizes BRCA-proficient cancer cells to PARP inhibitors

C Wang, L Tian, Q He, S Lin, Y Wu, Y Qiao, B Zhu, D Li… - Oncogene, 2023‏ - nature.com
Abstract Poly [ADP-ribose] polymerase (PARP) inhibitors, which selectively kills homologous
recombination (HR) repair-deficient cancer cells, are widely employed to treat cancer …

Dna2 removes toxic ssDNA-RPA filaments generated from meiotic recombination-associated DNA synthesis

B Zhai, S Zhang, B Li, J Zhang, X Yang… - Nucleic Acids …, 2023‏ - academic.oup.com
During the repair of DNA double-strand breaks (DSBs), de novo synthesized DNA strands
can displace the parental strand to generate single-strand DNAs (ssDNAs). Many …

Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA

DG Nickens, Z Feng, J Shen, SJ Gray… - Nucleic Acids …, 2024‏ - academic.oup.com
Telomerase is the enzyme that lengthens telomeres and is tightly regulated by a variety of
means to maintain genome integrity. Several DNA helicases function at telomeres, and we …