Single-cell transcriptomics reveals heterogeneity in plant responses to the environment: a focus on biotic and abiotic interactions

R Tenorio Berrío, M Dubois - Journal of Experimental Botany, 2024 - academic.oup.com
Biotic and abiotic environmental cues are major factors influencing plant growth and
productivity. Interactions with biotic (eg symbionts and pathogens) and abiotic (eg changes …

Lost in space: what single-cell RNA sequencing cannot tell you

K Adema, MA Schon, MD Nodine, W Kohlen - Trends in Plant Science, 2024 - cell.com
Plant scientists are rapidly integrating single-cell RNA sequencing (scRNA-seq) into their
workflows. Maximizing the potential of scRNA-seq requires a proper understanding of the …

Multiplexed single-cell 3D spatial gene expression analysis in plant tissue using PHYTOMap

T Nobori, M Oliva, R Lister, JR Ecker - Nature Plants, 2023 - nature.com
Retrieving the complex responses of individual cells in the native three-dimensional tissue
context is crucial for a complete understanding of tissue functions. Here, we present …

Single-nucleus transcriptomes reveal spatiotemporal symbiotic perception and early response in Medicago

Z Liu, J Yang, Y Long, C Zhang, D Wang, X Zhang… - Nature Plants, 2023 - nature.com
Establishing legume–rhizobial symbiosis requires precise coordination of complex
responses in a time-and cell type-specific manner. Encountering Rhizobium, rapid changes …

Best practices for the execution, analysis, and data storage of plant single-cell/nucleus transcriptomics

C Grones, T Eekhout, D Shi, M Neumann… - The Plant …, 2024 - academic.oup.com
Single-cell and single-nucleus RNA-sequencing technologies capture the expression of
plant genes at an unprecedented resolution. Therefore, these technologies are gaining …

Opportunities and challenges in the application of single-cell and spatial transcriptomics in plants

C Chen, Y Ge, L Lu - Frontiers in Plant Science, 2023 - frontiersin.org
Single-cell and spatial transcriptomics have diverted researchers' attention from the
multicellular level to the single-cell level and spatial information. Single-cell transcriptomes …

Cell biology of the leaf epidermis: Fate specification, morphogenesis, and coordination

DT Zuch, SM Doyle, M Majda, RS Smith… - The Plant …, 2022 - academic.oup.com
As the outermost layer of plants, the epidermis serves as a critical interface between plants
and the environment. During leaf development, the differentiation of specialized epidermal …

Single-nucleus sequencing deciphers developmental trajectories in rice pistils

C Li, S Zhang, X Yan, P Cheng, H Yu - Developmental Cell, 2023 - cell.com
Angiosperms possess a life cycle with an alternation of sporophyte and gametophyte
generations, which happens in plant organs like pistils. Rice pistils contain ovules and …

[HTML][HTML] Analysis of meristems and plant regeneration at single-cell resolution

RY Liao, JW Wang - Current opinion in plant biology, 2023 - Elsevier
Rapid development of high-throughput single-cell RNA sequencing (scRNA-seq)
technologies offers exciting opportunities to reveal new and rare cell types, previously …

AraLeTA: An Arabidopsis leaf expression atlas across diurnal and developmental scales

GYW Vong, K McCarthy, W Claydon, SJ Davis… - Plant …, 2024 - academic.oup.com
Mature plant leaves are a composite of distinct cell types, including epidermal, mesophyll,
and vascular cells. Notably, the proportion of these cells and the relative transcript …