Phylogenetic identification and in situ detection of individual microbial cells without cultivation

RI Amann, W Ludwig, KH Schleifer - Microbiological reviews, 1995 - Am Soc Microbiol
The frequent discrepancy between direct microscopic counts and numbers of culturable
bacteria from environmental samples is just one of several indications that we currently …

Metagenomics: application of genomics to uncultured microorganisms

J Handelsman - Microbiology and molecular biology reviews, 2004 - Am Soc Microbiol
Metagenomics (also referred to as environmental and community genomics) is the genomic
analysis of microorganisms by direct extraction and cloning of DNA from an assemblage of …

Archaea in coastal marine environments.

EF DeLong - Proceedings of the National Academy of …, 1992 - National Acad Sciences
Archaea (archaebacteria) are a phenotypically diverse group of microorganisms that share a
common evolutionary history. There are four general phenotypic groups of archaea: the …

The NIH human microbiome project

J Peterson, S Garges, M Giovanni, P McInnes… - Genome …, 2009 - genome.cshlp.org
The Human Microbiome Project (HMP), funded as an initiative of the NIH Roadmap for
Biomedical Research (http://nihroadmap. nih. gov), is a multi-component community …

Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity

P Hugenholtz, BM Goebel, NR Pace - Journal of bacteriology, 1998 - Am Soc Microbiol
Our perspective on microbial diversity has improved enormously over the past few decades.
In large part this has been due to molecular phylogenetic studies that objectively relate …

Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA

WT Liu, TL Marsh, H Cheng… - Applied and …, 1997 - Am Soc Microbiol
A quantitative molecular technique was developed for rapid analysis of microbial community
diversity in various environments. The technique employed PCR in which one of the two …

Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis

F v. Wintzingerode, UB Göbel… - FEMS microbiology …, 1997 - academic.oup.com
After nearly 10 years of PCR-based analysis of prokaryotic small-subunit ribosomal RNAs
for ecological studies it seems necessary to summarize reported pitfalls of this approach …

Bacterial rhodopsin: evidence for a new type of phototrophy in the sea

O Béja, L Aravind, EV Koonin, MT Suzuki, A Hadd… - Science, 2000 - science.org
Extremely halophilic archaea contain retinal-binding integral membrane proteins called
bacteriorhodopsins that function as light-driven proton pumps. So far, bacteriorhodopsins …

Environmental DNA: What's behind the term? Clarifying the terminology and recommendations for its future use in biomonitoring

J Pawlowski, L Apothéloz‐Perret‐Gentil… - Molecular …, 2020 - Wiley Online Library
The last decade brought a spectacular development of so‐called environmental (e) DNA
studies. In general,“environmental DNA” is defined as DNA isolated from environmental …

Prochlorococcus, a Marine Photosynthetic Prokaryote of Global Significance

F Partensky, WR Hess, D Vaulot - Microbiology and molecular …, 1999 - Am Soc Microbiol
The minute photosynthetic prokaryote Prochlorococcus, which was discovered about 10
years ago, has proven exceptional from several standpoints. Its tiny size (0.5 to 0.7 μm in …