Target identification of small molecules based on chemical biology approaches

Y Futamura, M Muroi, H Osada - Molecular BioSystems, 2013 - pubs.rsc.org
Recently, a phenotypic approach—screens that assess the effects of compounds on cells,
tissues, or whole organisms—has been reconsidered and reintroduced as a complementary …

The phosphate ester group in secondary metabolites

F Della-Felice, A de Andrade Bartolomeu… - Natural Product …, 2022 - pubs.rsc.org
Covering: 2000 to mid-2021 The phosphate ester is a versatile, widespread functional group
involved in a plethora of biological activities. Its presence in secondary metabolites …

Plant-derived antifungal agent poacic acid targets β-1, 3-glucan

JS Piotrowski, H Okada, F Lu, SC Li… - Proceedings of the …, 2015 - National Acad Sciences
A rise in resistance to current antifungals necessitates strategies to identify alternative
sources of effective fungicides. We report the discovery of poacic acid, a potent antifungal …

Vanillin Inhibits Translation and Induces Messenger Ribonucleoprotein (mRNP) Granule Formation in Saccharomyces cerevisiae: Application and Validation of High …

A Iwaki, S Ohnuki, Y Suga, S Izawa, Y Ohya - PLoS One, 2013 - journals.plos.org
Vanillin, generated by acid hydrolysis of lignocellulose, acts as a potent inhibitor of the
growth of the yeast Saccharomyces cerevisiae. Here, we investigated the cellular processes …

Defining Functions of Mannoproteins in Saccharomyces cerevisiae by High-Dimensional Morphological Phenoty**

F Ghanegolmohammadi, H Okada, Y Liu… - Journal of Fungi, 2021 - mdpi.com
Mannoproteins are non-filamentous glycoproteins localized to the outermost layer of the
yeast cell wall. The physiological roles of these structural components have not been …

Targeted mutations produce divergent characteristics in pedigreed sake yeast strains

N Klinkaewboonwong, S Ohnuki, T Chadani, I Nishida… - Microorganisms, 2023 - mdpi.com
Modification of the genetic background and, in some cases, the introduction of targeted
mutations can play a critical role in producing trait characteristics during the breeding of …

[HTML][HTML] Image analysis in microbiology: a review

E Puchkov - Journal of Computer and Communications, 2016 - scirp.org
This review is focused on using computer image analysis as a means of objective and
quantitative characterizing optical images of the macroscopic (eg microbial colonies) and …

High-throughput platform for yeast morphological profiling predicts the targets of bioactive compounds

S Ohnuki, I Ogawa, K Itto-Nakama, F Lu… - npj systems biology …, 2022 - nature.com
Morphological profiling is an omics-based approach for predicting intracellular targets of
chemical compounds in which the dose-dependent morphological changes induced by the …

Distinct roles of cell wall biogenesis in yeast morphogenesis as revealed by multivariate analysis of high-dimensional morphometric data

H Okada, S Ohnuki, C Roncero… - Molecular biology of …, 2014 - Am Soc Cell Biol
The cell wall of budding yeast is a rigid structure composed of multiple components. To
thoroughly understand its involvement in morphogenesis, we used the image analysis …

Phenotypic diagnosis of lineage and differentiation during sake yeast breeding

S Ohnuki, H Okada, A Friedrich, Y Kanno… - G3: Genes …, 2017 - academic.oup.com
Sake yeast was developed exclusively in Japan. Its diversification during breeding remains
largely uncharacterized. To evaluate the breeding processes of the sake lineage, we …