Phase separation as a missing mechanism for interpretation of disease mutations

B Tsang, I Pritišanac, SW Scherer, AM Moses… - Cell, 2020 - cell.com
It is unclear how disease mutations impact intrinsically disordered protein regions (IDRs),
which lack a stable folded structure. These mutations, while prevalent in disease, are …

Liquid-liquid phase separation in biology: mechanisms, physiological functions and human diseases

H Zhang, X Ji, P Li, C Liu, J Lou, Z Wang… - Science China Life …, 2020 - Springer
Cells are compartmentalized by numerous membrane-enclosed organelles and
membraneless compartments to ensure that a wide variety of cellular activities occur in a …

[HTML][HTML] Glycogen accumulation and phase separation drives liver tumor initiation

Q Liu, J Li, W Zhang, C **ao, S Zhang, C Nian, J Li… - Cell, 2021 - cell.com
Glucose consumption is generally increased in tumor cells to support tumor growth.
Interestingly, we report that glycogen accumulation is a key initiating oncogenic event during …

Synthetic protein condensates for cellular and metabolic engineering

ZG Qian, SC Huang, XX **a - Nature chemical biology, 2022 - nature.com
Protein condensates are distinct structures assembled in living cells that concentrate
molecules via phase separation in a confined subcellular compartment. In the past decade …

Properties and biological impact of RNA G-quadruplexes: from order to turmoil and back

P Kharel, G Becker, V Tsvetkov… - Nucleic acids research, 2020 - academic.oup.com
Abstract Guanine-quadruplexes (G4s) are non-canonical four-stranded structures that can
be formed in guanine (G) rich nucleic acid sequences. A great number of G-rich sequences …

Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses

F Geiger, J Acker, G Papa, X Wang, WE Arter… - The EMBO …, 2021 - embopress.org
RNA viruses induce the formation of subcellular organelles that provide microenvironments
conducive to their replication. Here we show that replication factories of rotaviruses …

Recent developments in the field of intrinsically disordered proteins: intrinsic disorder–based emergence in cellular biology in light of the physiological and …

VN Uversky - Annual Review of Biophysics, 2021 - annualreviews.org
This review deals with two important concepts—protein intrinsic disorder and proteinaceous
membrane-less organelles (PMLOs). The past 20 years have seen an upsurge of scientific …

Liquid-liquid phase separation in neuronal development and synaptic signaling

X Wu, Q Cai, Z Feng, M Zhang - Developmental cell, 2020 - cell.com
Formation of biomolecular condensates that are not enclosed by membranes via liquid-
liquid phase separation (LLPS) is a general strategy that cells adopt to organize …

Plants use molecular mechanisms mediated by biomolecular condensates to integrate environmental cues with development

S Field, GJ Jang, C Dean, LC Strader, SY Rhee - The Plant Cell, 2023 - academic.oup.com
This review highlights recent literature on biomolecular condensates in plant development
and discusses challenges for fully dissecting their functional roles. Plant developmental …

Essence determines phenomenon: assaying the material properties of biological condensates

Z Wang, J Lou, H Zhang - Journal of Biological Chemistry, 2022 - ASBMB
Intracellular spaces are partitioned into separate compartments to ensure that numerous
biochemical reactions and cellular functions take place in a spatiotemporally controlled …