The use of next generation sequencing for improving food safety: Translation into practice

B Jagadeesan, P Gerner-Smidt, MW Allard, S Leuillet… - Food …, 2019 - Elsevier
Abstract Next Generation Sequencing (NGS) combined with powerful bioinformatic
approaches are revolutionising food microbiology. Whole genome sequencing (WGS) of …

Metagenomics: the next culture-independent game changer

JD Forbes, NC Knox, J Ronholm, F Pagotto… - Frontiers in …, 2017 - frontiersin.org
A trend towards the abandonment of obtaining pure culture isolates in frontline laboratories
is at a crossroads with the ability of public health agencies to perform their basic mandate of …

Tracking a Hospital Outbreak of Carbapenem-Resistant Klebsiella pneumoniae with Whole-Genome Sequencing

ES Snitkin, AM Zelazny, PJ Thomas, F Stock… - Science translational …, 2012 - science.org
The Gram-negative bacteria Klebsiella pneumoniae is a major cause of nosocomial
infections, primarily among immunocompromised patients. The emergence of strains …

Implementation of nationwide real-time whole-genome sequencing to enhance listeriosis outbreak detection and investigation

BR Jackson, C Tarr, E Strain, KA Jackson… - Reviews of Infectious …, 2016 - academic.oup.com
Listeria monocytogenes (Lm) causes severe foodborne illness (listeriosis). Previous
molecular subty** methods, such as pulsed-field gel electrophoresis (PFGE), were critical …

Prospective Genomic Characterization of the German Enterohemorrhagic Escherichia coli O104:H4 Outbreak by Rapid Next Generation Sequencing Technology

A Mellmann, D Harmsen, CA Cummings, EB Zentz… - PloS one, 2011 - journals.plos.org
An ongoing outbreak of exceptionally virulent Shiga toxin (Stx)-producing Escherichia coli
O104: H4 centered in Germany, has caused over 830 cases of hemolytic uremic syndrome …

Use of whole-genome sequencing for food safety and public health in the United States

E Brown, U Dessai, S McGarry… - … pathogens and disease, 2019 - liebertpub.com
Whole-genome sequencing (WGS) is increasingly used by food regulatory and public health
agencies in the United States to facilitate the detection, investigation, and control of …

[HTML][HTML] CFSAN SNP Pipeline: an automated method for constructing SNP matrices from next-generation sequence data

S Davis, JB Pettengill, Y Luo, J Payne… - PeerJ Computer …, 2015 - peerj.com
The analysis of next-generation sequence (NGS) data is often a fragmented step-wise
process. For example, multiple pieces of software are typically needed to map NGS reads …

Rapid draft sequencing and real-time nanopore sequencing in a hospital outbreak of Salmonella

J Quick, P Ashton, S Calus, C Chatt, S Gossain… - Genome biology, 2015 - Springer
Abstract Background Foodborne outbreaks of Salmonella remain a pressing public health
concern. We recently detected a large outbreak of Salmonella enterica serovar Enteritidis …

Routine use of microbial whole genome sequencing in diagnostic and public health microbiology

CU Köser, MJ Ellington, EJP Cartwright, SH Gillespie… - 2012 - journals.plos.org
Whole genome sequencing (WGS) promises to be transformative for the practice of clinical
microbiology, and the rapidly falling cost and turnaround time mean that this will become a …

The present and future of whole genome sequencing (WGS) and whole metagenome sequencing (WMS) for surveillance of antimicrobial resistant microorganisms …

EA Oniciuc, E Likotrafiti, A Alvarez-Molina, M Prieto… - Genes, 2018 - mdpi.com
Antimicrobial resistance (AMR) surveillance is a critical step within risk assessment
schemes, as it is the basis for informing global strategies, monitoring the effectiveness of …