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The use of next generation sequencing for improving food safety: Translation into practice
Abstract Next Generation Sequencing (NGS) combined with powerful bioinformatic
approaches are revolutionising food microbiology. Whole genome sequencing (WGS) of …
approaches are revolutionising food microbiology. Whole genome sequencing (WGS) of …
Metagenomics: the next culture-independent game changer
A trend towards the abandonment of obtaining pure culture isolates in frontline laboratories
is at a crossroads with the ability of public health agencies to perform their basic mandate of …
is at a crossroads with the ability of public health agencies to perform their basic mandate of …
Tracking a Hospital Outbreak of Carbapenem-Resistant Klebsiella pneumoniae with Whole-Genome Sequencing
ES Snitkin, AM Zelazny, PJ Thomas, F Stock… - Science translational …, 2012 - science.org
The Gram-negative bacteria Klebsiella pneumoniae is a major cause of nosocomial
infections, primarily among immunocompromised patients. The emergence of strains …
infections, primarily among immunocompromised patients. The emergence of strains …
Implementation of nationwide real-time whole-genome sequencing to enhance listeriosis outbreak detection and investigation
Listeria monocytogenes (Lm) causes severe foodborne illness (listeriosis). Previous
molecular subty** methods, such as pulsed-field gel electrophoresis (PFGE), were critical …
molecular subty** methods, such as pulsed-field gel electrophoresis (PFGE), were critical …
Prospective Genomic Characterization of the German Enterohemorrhagic Escherichia coli O104:H4 Outbreak by Rapid Next Generation Sequencing Technology
A Mellmann, D Harmsen, CA Cummings, EB Zentz… - PloS one, 2011 - journals.plos.org
An ongoing outbreak of exceptionally virulent Shiga toxin (Stx)-producing Escherichia coli
O104: H4 centered in Germany, has caused over 830 cases of hemolytic uremic syndrome …
O104: H4 centered in Germany, has caused over 830 cases of hemolytic uremic syndrome …
Use of whole-genome sequencing for food safety and public health in the United States
E Brown, U Dessai, S McGarry… - … pathogens and disease, 2019 - liebertpub.com
Whole-genome sequencing (WGS) is increasingly used by food regulatory and public health
agencies in the United States to facilitate the detection, investigation, and control of …
agencies in the United States to facilitate the detection, investigation, and control of …
[HTML][HTML] CFSAN SNP Pipeline: an automated method for constructing SNP matrices from next-generation sequence data
S Davis, JB Pettengill, Y Luo, J Payne… - PeerJ Computer …, 2015 - peerj.com
The analysis of next-generation sequence (NGS) data is often a fragmented step-wise
process. For example, multiple pieces of software are typically needed to map NGS reads …
process. For example, multiple pieces of software are typically needed to map NGS reads …
Rapid draft sequencing and real-time nanopore sequencing in a hospital outbreak of Salmonella
Abstract Background Foodborne outbreaks of Salmonella remain a pressing public health
concern. We recently detected a large outbreak of Salmonella enterica serovar Enteritidis …
concern. We recently detected a large outbreak of Salmonella enterica serovar Enteritidis …
Routine use of microbial whole genome sequencing in diagnostic and public health microbiology
Whole genome sequencing (WGS) promises to be transformative for the practice of clinical
microbiology, and the rapidly falling cost and turnaround time mean that this will become a …
microbiology, and the rapidly falling cost and turnaround time mean that this will become a …
The present and future of whole genome sequencing (WGS) and whole metagenome sequencing (WMS) for surveillance of antimicrobial resistant microorganisms …
EA Oniciuc, E Likotrafiti, A Alvarez-Molina, M Prieto… - Genes, 2018 - mdpi.com
Antimicrobial resistance (AMR) surveillance is a critical step within risk assessment
schemes, as it is the basis for informing global strategies, monitoring the effectiveness of …
schemes, as it is the basis for informing global strategies, monitoring the effectiveness of …