Using chemical shift perturbation to characterise ligand binding

MP Williamson - Progress in nuclear magnetic resonance spectroscopy, 2013 - Elsevier
Chemical shift perturbation (CSP, chemical shift map** or complexation-induced changes
in chemical shift, CIS) follows changes in the chemical shifts of a protein when a ligand is …

Exploring free-energy landscapes of intrinsically disordered proteins at atomic resolution using NMR spectroscopy

MR Jensen, M Zweckstetter, J Huang… - Chemical …, 2014 - ACS Publications
The last 15 years have seen a paradigm shift in our understanding of protein biochemistry,
with the realization that an unexpectedly high fraction of the human genome codes for …

Water dispersion interactions strongly influence simulated structural properties of disordered protein states

S Piana, AG Donchev, P Robustelli… - The journal of physical …, 2015 - ACS Publications
Many proteins can be partially or completely disordered under physiological conditions.
Structural characterization of these disordered states using experimental methods can be …

A soluble α-synuclein construct forms a dynamic tetramer

W Wang, I Perovic, J Chittuluru… - Proceedings of the …, 2011 - National Acad Sciences
A heterologously expressed form of the human Parkinson disease-associated protein α-
synuclein with a 10-residue N-terminal extension is shown to form a stable tetramer in the …

Determination of secondary structure populations in disordered states of proteins using nuclear magnetic resonance chemical shifts

C Camilloni, A De Simone, WF Vranken… - Biochemistry, 2012 - ACS Publications
One of the major open challenges in structural biology is to achieve effective descriptions of
disordered states of proteins. This problem is difficult because these states are …

Sequence-specific random coil chemical shifts of intrinsically disordered proteins

K Tamiola, B Acar, FAA Mulder - Journal of the American …, 2010 - ACS Publications
Although intrinsically disordered proteins (IDPs) are widespread in nature and play diverse
and important roles in biology, they have to date been little characterized structurally …

Sequence correction of random coil chemical shifts: correlation between neighbor correction factors and changes in the Ramachandran distribution

M Kjaergaard, FM Poulsen - Journal of biomolecular NMR, 2011 - Springer
Random coil chemical shifts are necessary for secondary chemical shift analysis, which is
the main NMR method for identification of secondary structure in proteins. One of the largest …

pH-dependent random coil 1H, 13C, and 15N chemical shifts of the ionizable amino acids: a guide for protein pK a measurements

G Platzer, M Okon, LP McIntosh - Journal of biomolecular NMR, 2014 - Springer
The p K a values and charge states of ionizable residues in polypeptides and proteins are
frequently determined via NMR-monitored pH titrations. To aid the interpretation of the …

Random coil chemical shift for intrinsically disordered proteins: effects of temperature and pH

M Kjaergaard, S Brander, FM Poulsen - Journal of biomolecular NMR, 2011 - Springer
Secondary chemical shift analysis is the main NMR method for detection of transiently
formed secondary structure in intrinsically disordered proteins. The quality of the secondary …

Injectable tissue integrating networks from recombinant polypeptides with tunable order

S Roberts, TS Harmon, JL Schaal, V Miao, K Li… - Nature materials, 2018 - nature.com
Emergent properties of natural biomaterials result from the collective effects of nanoscale
interactions among ordered and disordered domains. Here, using recombinant sequence …