Based on technical advances in the sequencing and synthesis of genetic components as
well as the genome, significant progress has recently been made in develo** synthetic …

To promote sustainable development, the smart city implies a global vision that merges
artificial intelligence, big data, decision making, information and communication technology …

Abstract eQuilibrator (equilibrator. weizmann. ac. il) is a database of biochemical equilibrium
constants and Gibbs free energies, originally designed as a web-based interface. While the …

We present a whole-cell fully dynamical kinetic model (WCM) of JCVI-syn3A, a minimal cell
with a reduced genome of 493 genes that has retained few regulatory proteins or small …

Genome‐scale metabolic models (GEM s) are widely used to calculate metabolic
phenotypes. They rely on defining a set of constraints, the most common of which is that the …

Knowing the catalytic turnover numbers of enzymes is essential for understanding the
growth rate, proteome composition, and physiology of organisms, but experimental data on …

Increasing antibiotic resistance urges for new technologies for studying microbes and
antimicrobial mechanism of action. We adapted thermal proteome profiling (TPP) to probe …

Synthetic biology aims at designing new biological functions from first principles. These new
designs allow to expand the natural solution space and overcome the limitations of naturally …

Metabolic pathways reflect an organism's chemical repertoire and hence their elucidation
and design have been a primary goal in metabolic engineering. Various computational …

Enzymes catalyze a vast range of reactions. Their catalytic performances, mechanisms,
global folds, and active-site architectures are also highly diverse, suggesting that enzymes …