Nonlinear magic: multiphoton microscopy in the biosciences

WR Zipfel, RM Williams, WW Webb - Nature biotechnology, 2003‏ - nature.com
Multiphoton microscopy (MPM) has found a niche in the world of biological imaging as the
best noninvasive means of fluorescence microscopy in tissue explants and living animals …

Nonlinear optical microscopy: From fundamentals to applications in live bioimaging

V Parodi, E Jacchetti, R Osellame, G Cerullo… - … in bioengineering and …, 2020‏ - frontiersin.org
A recent challenge in the field of bioimaging is to image vital, thick, and complex tissues in
real time and in non-invasive mode. Among the different tools available for diagnostics …

Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure

X Chen, O Nadiarynkh, S Plotnikov, PJ Campagnola - Nature protocols, 2012‏ - nature.com
Second-harmonic generation (SHG) microscopy has emerged as a powerful modality for
imaging fibrillar collagen in a diverse range of tissues. Because of its underlying physical …

Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation

WR Zipfel, RM Williams, R Christie, AY Nikitin… - Proceedings of the …, 2003‏ - pnas.org
Multicolor nonlinear microscopy of living tissue using two-and three-photon-excited intrinsic
fluorescence combined with second harmonic generation by supermolecular structures …

[HTML][HTML] Interpreting second-harmonic generation images of collagen I fibrils

RM Williams, WR Zipfel, WW Webb - Biophysical journal, 2005‏ - cell.com
Fibrillar collagen, being highly noncentrosymmetric, possesses a tremendous nonlinear
susceptibility. As a result, second-harmonic generation (SHG) microscopy of collagen …

Noninvasive assessment of collagen gel microstructure and mechanics using multiphoton microscopy

CB Raub, V Suresh, T Krasieva, J Lyubovitsky, JD Mih… - Biophysical journal, 2007‏ - cell.com
Multiphoton microscopy of collagen hydrogels produces second harmonic generation (SHG)
and two-photon fluorescence (TPF) images, which can be used to noninvasively study gel …

Second harmonic generation microscopy: principles and applications to disease diagnosis

PJ Campagnola, CY Dong - Laser & Photonics Reviews, 2011‏ - Wiley Online Library
In this review, the authors discuss the underlying principles of SHG and specific factors that
affect the generation properties and describe the essential components of a SHG instrument …

Second harmonic spectroscopy of aqueous nano-and microparticle interfaces

KB Eisenthal - Chemical reviews, 2006‏ - ACS Publications
Applications of the interface-selective methods of second harmonic (SHG) and sum
frequency (SFG) spectroscopy to the investigation of equilibrium and time-dependent …

[ספר][B] Second-order nonlinear optical characterization techniques: an introduction

T Verbiest, K Clays, V Rodriguez - 2009‏ - taylorfrancis.com
Although chemists, biochemists, biologists, and material scientists are often interested in
using nonlinear optical techniques for characterizing their samples, they seldom have the …

Second harmonic generation imaging of collagen fibrils in cornea and sclera

M Han, G Giese, JF Bille - Optics express, 2005‏ - opg.optica.org
Collagen, as the most abundant protein in the human body, determines the unique
physiological and optical properties of the connective tissues including cornea and sclera …