The Mediator complex, which in humans is 1.4 MDa in size and includes 26 subunits,
controls many aspects of RNA polymerase II (Pol II) function. Apart from its size, a defining …

Chemical modifications on mRNA represent a critical layer of gene expression regulation.
Research in this area has continued to accelerate over the last decade, as more …

The CORUM database has been providing comprehensive reference information about
experimentally characterized, mammalian protein complexes and their associated biological …

Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era.
We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data …

The IntAct molecular interaction database (https://www. ebi. ac. uk/intact) is a curated
resource of molecular interactions, derived from the scientific literature and from direct data …

Rho GTPases are a family of small G proteins that regulate a wide array of cellular
processes related to their key roles controlling the cytoskeleton. Cancer is a multistep …

Pooled CRISPR screens with single-cell RNA sequencing readout (Perturb-seq) have
emerged as a key technique in functional genomics, but they are limited in scale by cost and …

Defining the cellular response to pharmacological agents is critical for understanding the
mechanism of action of small molecule perturbagens. Here, we developed a 96-well-plate …

Cells use ubiquitin to mark proteins for proteasomal degradation. Although the proteasome
also eliminates proteins that are not ubiquitinated, how this occurs mechanistically is …

Clustered regularly interspaced short palindromic repeat interference (CRISPRi), based on
the fusion of inactive Cas9 (dCas9) to the Krüppel-associated box (KRAB) repressor, is a …