Tissue clearing to examine tumour complexity in three dimensions

J Almagro, HA Messal, M Zaw Thin… - Nature Reviews …, 2021 - nature.com
The visualization of whole organs and organisms through tissue clearing and fluorescence
volumetric imaging has revolutionized the way we look at biological samples. Its application …

Biomedical applications of tissue clearing and three-dimensional imaging in health and disease

MV Gomez-Gaviro, D Sanderson, J Ripoll, M Desco - Iscience, 2020 - cell.com
Summary Three-dimensional (3D) optical imaging techniques can expand our knowledge
about physiological and pathological processes that cannot be fully understood with 2D …

Intraclonal plasticity in mammary tumors revealed through large-scale single-cell resolution 3D imaging

AC Rios, BD Capaldo, F Vaillant, B Pal, R van Ineveld… - Cancer cell, 2019 - cell.com
Breast tumors are inherently heterogeneous, but the evolving cellular organization through
neoplastic progression is poorly understood. Here we report a rapid, large-scale single-cell …

Hydrogel-tissue chemistry: principles and applications

V Gradinaru, J Treweek, K Overton… - Annual review of …, 2018 - annualreviews.org
Over the past five years, a rapidly develo** experimental approach has enabled high-
resolution and high-content information retrieval from intact multicellular animal (metazoan) …

CUBIC pathology: three-dimensional imaging for pathological diagnosis

S Nojima, EA Susaki, K Yoshida, H Takemoto… - Scientific reports, 2017 - nature.com
The examination of hematoxylin and eosin (H&E)-stained tissues on glass slides by
conventional light microscopy is the foundation for histopathological diagnosis. However …

CSF1R-dependent macrophages control postnatal somatic growth and organ maturation

S Keshvari, M Caruso, N Teakle, L Batoon… - PLoS …, 2021 - journals.plos.org
Homozygous mutation of the Csf1r locus (Csf1rko) in mice, rats and humans leads to
multiple postnatal developmental abnormalities. To enable analysis of the mechanisms …

Multiscale imaging of basal cell dynamics in the functionally mature mammary gland

AJ Stevenson, G Vanwalleghem… - Proceedings of the …, 2020 - National Acad Sciences
The mammary epithelium is indispensable for the continued survival of more than 5,000
mammalian species. For some, the volume of milk ejected in a single day exceeds their …

Advances in ex situ tissue optical clearing

P Matryba, L Kaczmarek, J Gołąb - Laser & Photonics Reviews, 2019 - Wiley Online Library
Examination of whole organs with subcellular resolution in health, disease, and during
development is necessary to decipher their biological complexity. However, until recently …

Mammary stem cells: premise, properties, and perspectives

B Lloyd-Lewis, OB Harris, CJ Watson, FM Davis - Trends in cell biology, 2017 - cell.com
Adult mammary stem cells (MaSCs) drive postnatal organogenesis and remodeling in the
mammary gland, and their longevity and potential have important implications for breast …

Imaging the invisible—Bioorthogonal Raman probes for imaging of cells and tissues

G Azemtsop Matanfack, J Rüger… - Journal of …, 2020 - Wiley Online Library
A revolutionary avenue for vibrational imaging with super‐multiplexing capability can be
seen in the recent development of Raman‐active bioortogonal tags or labels. These tags …