Metabolic engineering aims to develop efficient cell factories by rewiring cellular
metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae …

Coupling the tools of synthetic biology with traditional molecular genetic techniques can
enable the rapid prototy** and optimization of yeast strains. While the era of yeast …

Abstract Design of de novo synthetic regulatory DNA is a promising avenue to control gene
expression in biotechnology and medicine. Using mutagenesis typically requires screening …

Saccharomyces cerevisiae is an increasingly attractive host for synthetic biology because of
its long history in industrial fermentations. However, until recently, most synthetic biology …

Understanding the genetic regulatory code governing gene expression is an important
challenge in molecular biology. However, how individual coding and non-coding regions of …

Cells can be programmed to monitor and react to their environment using genetic circuits.
Design automation software maps a desired circuit function to a DNA sequence, a process …

CO2 fixation plays a key role to make biobased production cost competitive. Here, we use 3-
hydroxypropionic acid (3-HP) to showcase how CO2 fixation enables approaching …

Engineered genetic systems are prone to failure when their genetic parts contain repetitive
sequences. Designing many nonrepetitive genetic parts with desired functionalities remains …

Synthetic promoters, especially minimally sized, are critical for advancing fungal synthetic
biology. Fungal promoters often span hundreds of base pairs, nearly ten times the amount of …

Synthetic biology continues to progress by relying on more robust tools for transcriptional
control, of which promoters are the most fundamental component. Numerous studies have …