Clonal populations of microbial cells often show a high degree of phenotypic variability
under homogeneous conditions. Stochastic fluctuations in the cellular components that …

Synthetic control of gene expression is critical for metabolic engineering efforts. Specifically,
precise control of key pathway enzymes (heterologous or native) can help maximize product …

Understanding the genetic regulatory code governing gene expression is an important
challenge in molecular biology. However, how individual coding and non-coding regions of …

Heterologous pathways used in metabolic engineering may produce intermediates toxic to
the cell. Dynamic control of pathway enzymes could prevent the accumulation of these …

We have generated a series of variable-strength, constitutive, bacterial promoters that act
predictably in different sequence contexts, span two orders of magnitude in strength and …

Well-characterized promoters are essential tools for metabolic engineering and synthetic
biology. In Streptomyces coelicolor, the native kasO p is a temporally expressed promoter …

Engineering artificial gene networks from modular components is a major goal of synthetic
biology. However, the construction of gene networks with predictable functions remains …

Metabolic pathway engineering in the yeast Saccharomyces cerevisiae leads to improved
production of a wide range of compounds, ranging from ethanol (from biomass) to natural …

The soil bacterium Pseudomonas putida is increasingly attracting considerable interest as a
platform for advanced metabolic engineering through synthetic biology approaches …

Terpenoids constitute a large and diverse class of natural products that serve many
functions in nature. Most of the tens of thousands of the discovered terpenoids are …